Summary of Study ST002527
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002527 |
| Study Title | HPLC-MS-MS analysis amino acid levels in PDAC IF samples upon arginase inhibition |
| Study Summary | To test the hypothesis that myeloid arginase-1 activity could be responsible for pancreatic ductal adenocarninoma microenvironmental arginine starvation (PMID: 30990168), we generated orthotopic allograft mPDAC tumors in a mouse model with myeloid specific Arg1 knockout (LysM-Cre+/+-; Arg1fl/fl) and control animals (Arg1fl/fl). We also tested this with pharmacological inhibition of arginase-1 with the small-molecule inhibitor CB-1158. We then isolated IF from these tumors at end-stage and measured the levels of amino acids including arginine and ornithine in these samples. |
| Institute | University of Chicago |
| Last Name | Apiz Saab |
| First Name | Juan |
| Address | 929 E. 57th St. |
| japizsaab@uchicago.edu | |
| Phone | 7738346506 |
| Submit Date | 2022-08-05 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004161 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak area (m/z) |
MS:
| MS ID: | MS003908 |
| Analysis ID: | AN004161 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | In positive/negative polarity switching mode, an m/z scan range from 60 to 900 was chosen and MS1 data was collected at a resolution of 70,000. The automatic gain control (AGC) target was set at 1 × 106 and the maximum injection time was 200 ms. The targeted ions were subsequently fragmented, using the higher energy collisional dissociation (HCD) cell set to 30% normalized collision energy in MS2 at a resolution power of 17,500. Besides matching m/z, target metabolites are identified by matching either retention time with analytical standards and/or MS2 fragmentation pattern. Data acquisition and analysis were carried out by Xcalibur 4.1 software and Tracefinder 4.1 software, respectively (both from Thermo Fisher Scientific). |
| Ion Mode: | UNSPECIFIED |
