Summary of Study ST001644
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001052. The data can be accessed directly via it's Project DOI: 10.21228/M8QD76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001644 |
Study Title | In Vitro Characterization and Metabolomic Analysis of Cold-Stored Platelets |
Study Summary | Platelet concentrates are currently stored at room temperature (RPs) under constant agitation for up to 5-7 days depending on national regulations. However, platelet quality deteriorates during storage and room temperature storage also increases the risk of bacterial growth. Previous studies have shown that cold-stored platelets (CPs) have higher hemostatic function and can be stored for up to three weeks. While these studies have compared the metabolic phenotypes of CPs and RPs, they have not compared the impact of storage temperature and cold agitation (CPAs) on platelet function, nor have they identified metabolic correlates to such parameters. In vitro analysis showed CPAs and CPs had reduced count, faster CD62P expression and increased lactadherin binding. Furthermore, CPAs and CPs had higher maximal aggregation and a reduced aggregation lag phase compared to RPs. Metabolomic analysis revealed CPAs and CPs exhibited lower oxidative stress shown by preserved glutathione and pentose phosphate pools. CPAs and CPs also had reduced markers of beta-oxidation and amino acid catabolism demonstrating reduced needs for energy. Agitation did not significantly impact in vitro function or metabolomic parameters of cold-stored platelets. Correlation of in vitro and metabolomic results highlighted important metabolites that may contribute to stored platelet functions. |
Institute | University of Colorado Anschutz Medical Campus |
Department | Biochemistry and Molecular Genetics |
Laboratory | Angelo D'Alessandro |
Last Name | D'Alessandro |
First Name | Angelo |
Address | 12801 E 17th Ave L18-9403D |
angelo.dalessandro@cuanschutz.edu | |
Phone | 3037245798 |
Submit Date | 2021-01-08 |
Num Groups | 3 |
Total Subjects | 8 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-01-25 |
Release Version | 1 |
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Project:
Project ID: | PR001052 |
Project DOI: | doi: 10.21228/M8QD76 |
Project Title: | In Vitro Characterization and Metabolomic Analysis of Cold-Stored Platelets |
Project Summary: | Platelet concentrates are currently stored at room temperature (RPs) under constant agitation for up to 5-7 days depending on national regulations. However, platelet quality deteriorates during storage and room temperature storage also increases the risk of bacterial growth. Previous studies have shown that cold-stored platelets (CPs) have higher hemostatic function and can be stored for up to three weeks. While these studies have compared the metabolic phenotypes of CPs and RPs, they have not compared the impact of storage temperature and cold agitation (CPAs) on platelet function, nor have they identified metabolic correlates to such parameters. In vitro analysis showed CPAs and CPs had reduced count, faster CD62P expression and increased lactadherin binding. Furthermore, CPAs and CPs had higher maximal aggregation and a reduced aggregation lag phase compared to RPs. Metabolomic analysis revealed CPAs and CPs exhibited lower oxidative stress shown by preserved glutathione and pentose phosphate pools. CPAs and CPs also had reduced markers of beta-oxidation and amino acid catabolism demonstrating reduced needs for energy. Agitation did not significantly impact in vitro function or metabolomic parameters of cold-stored platelets. Correlation of in vitro and metabolomic results highlighted important metabolites that may contribute to stored platelet functions. |
Institute: | University of Colorado Anschutz Medical Campus |
Department: | Biochemistry and Molecular Genetics |
Laboratory: | Angelo D'Alessandro |
Last Name: | D'Alessandro |
First Name: | Angelo |
Address: | 12801 E 17th Ave L18-9403D, Aurora, Colorado, 80045, USA |
Email: | angelo.dalessandro@cuanschutz.edu |
Phone: | 303-724-5798 |