Summary of Study ST002051
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001297. The data can be accessed directly via it's Project DOI: 10.21228/M82H7B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002051 |
Study Title | The apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. |
Study Summary | The apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. Here, we developed a human myotube-based in vitro culture model of functionally mature tissue cysts. Metabolomic characterization of purified cysts reveals global changes that comprise increased levels of amino acids and decreased abundance of nucleobase- and tricarboxylic acid cycle-associated metabolites. In contrast to fast replicating tachyzoite forms of T. gondii these tissue cysts tolerate exposure to the aconitase inhibitor sodium fluoroacetate. |
Institute | Robert Koch-Institute |
Department | NG2 |
Laboratory | NG2 |
Last Name | Blume |
First Name | Martin |
Address | Seestraße 10 |
blumem@rki.de | |
Phone | +49 30 18754 2572 |
Submit Date | 2022-01-04 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2022-02-28 |
Release Version | 1 |
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Project:
Project ID: | PR001297 |
Project DOI: | doi: 10.21228/M82H7B |
Project Title: | In vitro maturation of Toxoplasma gondii bradyzoites in human myotubes and their metabolomic characterization |
Project Type: | characterization of in vitro T. gondii stages |
Project Summary: | The apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. Here, we developed a human myotube-based in vitro culture model of functionally mature tissue cysts. Metabolomic characterization of purified cysts reveals global changes that comprise increased levels of amino acids and decreased abundance of nucleobase- and tricarboxylic acid cycle-associated metabolites. In contrast to fast replicating tachyzoite forms of T. gondii these tissue cysts tolerate exposure to the aconitase inhibitor sodium fluoroacetate. |
Institute: | Robert Koch-Institut |
Last Name: | Blume |
First Name: | Martin |
Address: | Seestr. 10, Berlin, Berlin, 13353, Germany |
Email: | blumem@rki.de |
Phone: | +49 30 18754 2572 |