Summary of Study ST000315

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000254. The data can be accessed directly via it's Project DOI: 10.21228/M8Z88C This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000315
Study Title	Metabolomics and Childhood Obesity: A Pilot and Feasibility Study With Multiple Phenotypic Anchors
Study Type	Metabolomics
Study Summary	“Metabolomics” is a powerful new analytical approach for measuring and evaluating all small and intermediate sized metabolites in a variety of tissues or samples in conditions of health and disease. The purpose of this research is to determine if “metabolomics” can be used to address several important unanswered questions about obesity in children. First we will use metabolomics to identify patterns of metabolites in blood that are unique to obese children. We will then determine if these patterns are predictive of excessive weight gain and/or poor weight loss response in non-obese and obese children enrolled in an exercise program.
Institute	University of North Carolina
Department	Systems and Translational Sciences
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2015-12-31
Total Subjects	219
Raw Data Available	Yes
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2016-12-31
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000343
Sampleprep Summary:	Plasma samples were transferred to labeled tubes. A total of 219 study samples were thawed on ice for sample preparation200 uL of plasma sample were thawed and transferred to labeled tubes on ice where they were mixed with 50 uL Saline master mix (5mM Formate). Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 25 µL of each sample of each respective phenotypical experimental sample into different 1.5 mL tubes. Whole study (total) pools were generated by transferring 200 uL of plasma from each Pool sample into a 2.0 mL tube. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000343

Sampleprep Summary:

Plasma samples were transferred to labeled tubes. A total of 219 study samples were thawed on ice for sample preparation200 uL of plasma sample were thawed and transferred to labeled tubes on ice where they were mixed with 50 uL Saline master mix (5mM Formate). Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 25 µL of each sample of each respective phenotypical experimental sample into different 1.5 mL tubes. Whole study (total) pools were generated by transferring 200 uL of plasma from each Pool sample into a 2.0 mL tube. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer.