Summary of Study ST000414

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000323. The data can be accessed directly via it's Project DOI: 10.21228/M8ZS3N This work is supported by NIH grant, U2C- DK119886.


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Study IDST000414
Study TitleMetabolomics-based screening of the Malaria Box reveals both novel and established mechanisms of action
Study TypeDrug incubation
Study SummaryMeasuring the impact of MMV Malaria Box compounds on metabolism in Plasmodium falciparum-infected red blood cells
Monash Institute of Pharmaceutical Sciences
DepartmentDrug delivery, disposition and dynamics
LaboratoryCreek lab
Last NameCreek
First NameDarren
Address381 Royal Parade, Parkville, Melbourne, VIC3052, Australia
Submit Date2016-06-16
Num Groups126
Total Subjects18
Study Comments524
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Darren Creek Darren Creek application/zip

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Sample Preparation:

Sampleprep ID:SP000442
Sampleprep Summary:Culture medium was removed by aspiration, and the metabolism of the settled iRBCs quenched by addition of ice-cold phosphate buffered saline (PBS). Subsequent steps were performed on ice. Cells were pelleted by centrifugation for 5 min at 1000 × g and the PBS supernatant removed prior to the addition of 135 µL methanol (containing internal standard compounds: CHAPS, CAPS and PIPES) and rapid mixing by pipetting three times to extract iRBC metabolites. Samples were left on ice with gentle agitation for 60 minutes, then centrifuged at 3000 × g to remove the insoluble material. Supernatants were transferred to glass HPLC vials and stored (< 4 months) at -80 °C until analysis.
Processing Method:Lysis with organic solvent and mixing at 4°C
Processing Storage Conditions:on ice or 4°C
Extraction Method:Methanol (10:1)
Extract Storage:-80°C
Sample Spiking:internal standards (CHAPS, CAPS, PIPES all at 1 µM) mixed in extraction solvent
Cell Type:Plasmodium falciparum-infected red blood cells (8% parasitaemia and 3% haematocrit