Summary of Study ST001038
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000695. The data can be accessed directly via it's Project DOI: 10.21228/M8V100 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001038 |
| Study Title | Global Metabolomics of the Placenta Reveals Distinct Metabolic Profiles between Maternal and Fetal Placental Tissues Following Delivery in Non-Labored Women |
| Study Type | Timecourse |
| Study Summary | We evaluated the metabolic alterations in maternal and fetal placental tissues from non-labored women undergoing cesarean section using samples collected from 5 min to 24 h following delivery. Using 1H-NMR, we identified 14 metabolites that significantly differed between maternal and fetal placental tissues (FDR-corrected p-value < 0.05), with 12 metabolites elevated in the maternal tissue, reflecting the flux of these metabolites from mother to fetus. In the maternal tissue, 4 metabolites were significantly altered at 15 min, 10 metabolites at 30 min, and 16 metabolites at 1 h postdelivery, while 11 metabolites remained stable over 24 h. In contrast, in the fetal placenta tissue, 1 metabolite was significantly altered at 15 min, 2 metabolites at 30 min, and 4 metabolites at 1 h postdelivery, while 22 metabolites remained stable over 24 h. Our study provides information on the metabolic profiles of maternal and fetal placental tissues delivered by cesarean section and reveals that there are different metabolic alterations in the maternal and fetal tissues of the placenta following delivery. |
| Institute | University of Florida |
| Department | Biochemistry & Molecular Biology |
| Last Name | Jacquelyn |
| First Name | Walejko |
| Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| jwalejko@ufl.edu | |
| Phone | NA |
| Submit Date | 2018-08-20 |
| Num Groups | 10 |
| Total Subjects | 226 |
| Publications | doi: 10.3390/metabo8010010 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2018-10-10 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001084 |
| Sampleprep Summary: | The placental tissue samples (24.15–192.21 mg; mean 126.21 mg) were cut on dry ice and washed with ice-cold 0.85% sodium chloride to remove the excess blood before being placed in prechilled tubes. Five hundred μL of ice-cold 50/50 methanol/water was added to each sample before vortexing for 1 min. Homogenization and sonication methods were compared for metabolite extractions. Equivalent results were observed for both methods and, therefore, sonication was used because of its ability for high-throughput sample preparation. The samples were sonicated for 20 min to extract the metabolites and then spun at 14,000 rcf for 15 min at 4 °C. Four hundred and fifty μL of supernatant was transferred to a new microcentrifuge tube and concentrated overnight using a CentriVap Benchtop Vacuum Concentrator (Labconco, Kanas City, MO, USA). The samples were frozen at −80 °C until metabolomics analysis. he concentrated tissue specimens were thawed, reconstituted in 600 μL of 100 mM sodium phosphate buffer at pH 7.0, and vortexed until the pellets dissolved. The samples were centrifuged at 14,000 rcf for 15 min at 4 °C before transferring 590 μL into 5 mm NMR tubes (Bruker Biospin, USA). Organ: Placenta, Maternal and fetal surfaces |
| Processing Method: | Sonication and solvent removal w/Speed Vac |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 1:1 MeOH/H2O |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 600uL of 100mM sodium phosphate buffer at pH of 7.0 |
