Summary of Study ST001110
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000741. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ2Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001110 |
| Study Title | Investigating link between metabolism and circadian rhythm in Drosophila melanogaster |
| Study Summary | We are investigating the link between metabolism, circadian rhythm, and a high-fat diet with an emphasis on the role of metabolites that affect protein post translational modifications. We've hypothesized that protein modifications, which are critical to circadian clock functions, can be affected by different metabolic profiles, such as an excess or lack of fat in the diet, that ultimately regulate changes in circadian biology. |
| Institute | University of California, Davis |
| Department | College of Biological Sciences |
| Last Name | Contreras |
| First Name | Adam |
| Address | 202 Life Sciences Building, Kleiber Hall Drive, Davis, CA, 95616 |
| ajcon@ucdavis.edu | |
| Phone | 512-393-4773 |
| Submit Date | 2018-10-19 |
| Total Subjects | 96 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001162 |
| Sampleprep Summary: | Add 1 mL of 3:3:2 Acetonitrile:Isopropyl Alcohol:LC-MS Grade Water to each preweighed sample. Vortex each sample for 5 seconds. Shake at 4°C for 5 minutes using the Orbital Mixing Chilling/Heating Plate. Keep remaining samples on ice before shaking. Centrifuge samples for 2 minutes at 14000 rcf. Aliquot two 450 µL portions of the supernatant. Submit one to analysis and save one as backup at -20°C. To the submitted sample, dry in Labconco Centrivap. Submit to derivatization. |
| Sampleprep Protocol Filename: | SOP_blood-GCTOF-11082012_332.pdf |
| Sampleprep Protocol Comments: | Extraction solvent is prepared previously. Begin procedure at Sample Preparation under step 3. |
| Sample Derivatization: | Add 10 µL of prepared MeOX solution to each sample. Shake for 1.5 hours at 30°C. Add 91 µL of prepared MSTFA + FAME solution to each sample. Shake for 30 minutes at 37°C. |
