Summary of Study ST001270
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000855. The data can be accessed directly via it's Project DOI: 10.21228/M85H5H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001270 |
| Study Title | Necrotizing soft-tissue infections (NSTIs) metabolomics |
| Study Summary | Necrotizing soft-tissue infections (NSTIs) have multiple causes, risk factors, anatomical locations, and pathogenic mechanisms. In patients with NSTI, circulating metabolites may serve as substrate having impact on bacterial adaptation at the site of infection. Metabolic signatures associated with NSTI may reveal potential be useful as diagnostic and prognostic markers, as well as novel targets for therapy. This study used untargeted metabolomics analyses of plasma from NSTI patients (n=34) and healthy (non-infected) controls (n=24) to identify the metabolic signatures and connectivity patterns among metabolites associated with NSTI. |
| Institute | Wageningen University & Research |
| Last Name | Edoardo |
| First Name | Saccenti |
| Address | Stippeneng 4, 6708 Wageningen, the Netherlands |
| edoardo.saccenti@wur.nl | |
| Phone | +31644482628 |
| Submit Date | 2019-10-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2020-01-06 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001346 |
| Sampleprep Summary: | GCMS analysis was performed as described previously (A et al. 2005; Gullberg et al. 2004). In detail, 1 μL of the derivatized sample was injected in splitless mode by a CTC Combi Pal autosampler (CTC Analytics AG, Switzerland) into an Agilent 6890 gas chromatograph equipped with a 10 m x 0.18 mm fused silica capillary column with a chemically bonded 0.18 μm DB 5-MS UI stationary phase (J&W Scientific). The injector temperature was 270 °C, the purge flow rate was 20 mL min-1 and the purge was turned on after 60 seconds. The gas flow rate through the column was 1 mL min-1, the column temperature was held at 70 °C for 2 minutes, then increased by 40 °C min-1 to 320 °C, and held there for 2 minutes. The column effluent was introduced into the ion source of a Pegasus III time-of-flight mass spectrometer, GC/TOFMS (Leco Corp., St Joseph, MI, USA). The transfer line and the ion source temperatures were 250 °C and 200 °C, respectively. Ions were generated by a 70 eV electron beam at an ionization current of 2.0 mA, and 30 spectra s-1 were recorded in the mass range m/z 50 - 800. The acceleration voltage was turned on after a solvent delay of 150 seconds. The detector voltage was 1500-2000 V. |
| Sampleprep Protocol ID: | Analytical_parameters_Sleipner_10m_column_P0287_plasma.pdf |
