Summary of Study ST002200

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001403. The data can be accessed directly via it's Project DOI: 10.21228/M8C99T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002200
Study Title	Human Trisome Project Plasma Metabolomics v1
Study Summary	Analysis of metabolite relative abundance in blood plasma from 316 individuals with trisomy 21 (T21, Down syndrome) and 103 euploid controls. This dataset is part of the Human Trisome Project run by the Linda Crnic Institute for Down Syndrome at the University of Colorado Anschutz Medical Campus. http://www.trisome.org/
Institute	University of Colorado Denver
Laboratory	PIs - Joaquin Espinosa and Angelo D'Alessandro
Last Name	Haines
First Name	Julie
Address	12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA
Email	julie.haines@cuanschutz.edu
Phone	3037243339
Submit Date	2022-06-15
Num Groups	2
Total Subjects	419
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2023-06-15
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002292
Sampleprep Summary:	Metabolite extraction: Samples were thawed on ice and 20 μL of sample was diluted in 480 μL of LC-MS grade cold methanol/acetonitrile/water (5:3:2). Samples were incubated at 4°C for 30 min with vigorous vortexing. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 50 uL aliquots were transferred to autosampler vials. Lipid extraction: Samples were thawed on ice and 10 uL of sample was diluted in 90 uL of LC-MS grade cold methanol. Samples were briefly vortexed to mix then incubated for 30 min at -20C. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 25 uL aliquots were transferred to autosampler vials and diluted with 25 uL of 10 mM ammonium acetate.

Sampleprep ID:

SP002292

Sampleprep Summary:

Metabolite extraction: Samples were thawed on ice and 20 μL of sample was diluted in 480 μL of LC-MS grade cold methanol/acetonitrile/water (5:3:2). Samples were incubated at 4°C for 30 min with vigorous vortexing. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 50 uL aliquots were transferred to autosampler vials. Lipid extraction: Samples were thawed on ice and 10 uL of sample was diluted in 90 uL of LC-MS grade cold methanol. Samples were briefly vortexed to mix then incubated for 30 min at -20C. Supernatants were clarified by centrifugation (10 min, 18,213 g, 4C) then 25 uL aliquots were transferred to autosampler vials and diluted with 25 uL of 10 mM ammonium acetate.