Summary of Study ST002491

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001608. The data can be accessed directly via it's Project DOI: 10.21228/M8VB03 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002491
Study Title	Six tryptophan metabolites in mouse serum
Study Summary	We establish that six tryptophan metabolites are essentially not CYP1A1 substrates and the level of these metabolite in mouse serum are not affected by a lack of CYP1A1 or any other Aryl hydrocarbon Receptor (AHR) dependent metabolic pathway expression in vivo. These results establish that tryptophan metabolites that circulate at significant levels in vivo are not subject to an autoregulatory feedback loop between the AHR and CYP1A1.
Institute	Pennsylvania State University
Last Name	Dong
First Name	Fancong
Address	309 Life Sciences Building, University Park, PA 16802
Email	fxd93@psu.edu
Phone	8148651415
Submit Date	2023-02-22
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-04-03
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002587
Sampleprep Summary:	C57BL6/J mice were obtained from Jackson Laboratory (Bar Harbor, ME), bred in-house and maintained on a chow diet. Ahr+/-, and Ahr-/- mice were kindly provided by Dr. Christopher Bradfield (University of Wisconsin-Madison) and bred using female Ahr+/- and male Ahr-/- mice. Mice were housed on corncob bedding in a temperature- and light-controlled facility and given access to food and water ad libitum. Mice were also maintained in a pathogen-free facility and treated humanely with approval from the Animal Care and Use Committee of the Pennsylvania State University and methods were carried out in accordance with approved guidelines. Mice in the 8-10-week-old range were utilized in all experiments. Serum samples obtained from mice exposed to PCB126 for 5 days was previously described. Briefly, mice were fed dough pills once every 24 h, each pill contained 24 µg/kg PCB126 for five days and mice were sacrificed on day 6.

Sampleprep ID:

SP002587

Sampleprep Summary:

C57BL6/J mice were obtained from Jackson Laboratory (Bar Harbor, ME), bred in-house and maintained on a chow diet. Ahr+/-, and Ahr-/- mice were kindly provided by Dr. Christopher Bradfield (University of Wisconsin-Madison) and bred using female Ahr+/- and male Ahr-/- mice. Mice were housed on corncob bedding in a temperature- and light-controlled facility and given access to food and water ad libitum. Mice were also maintained in a pathogen-free facility and treated humanely with approval from the Animal Care and Use Committee of the Pennsylvania State University and methods were carried out in accordance with approved guidelines. Mice in the 8-10-week-old range were utilized in all experiments. Serum samples obtained from mice exposed to PCB126 for 5 days was previously described. Briefly, mice were fed dough pills once every 24 h, each pill contained 24 µg/kg PCB126 for five days and mice were sacrificed on day 6.