Summary of Study ST002491

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001608. The data can be accessed directly via it's Project DOI: 10.21228/M8VB03 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002491
Study Title	Six tryptophan metabolites in mouse serum
Study Summary	We establish that six tryptophan metabolites are essentially not CYP1A1 substrates and the level of these metabolite in mouse serum are not affected by a lack of CYP1A1 or any other Aryl hydrocarbon Receptor (AHR) dependent metabolic pathway expression in vivo. These results establish that tryptophan metabolites that circulate at significant levels in vivo are not subject to an autoregulatory feedback loop between the AHR and CYP1A1.
Institute	Pennsylvania State University
Last Name	Dong
First Name	Fancong
Address	309 Life Sciences Building, University Park, PA 16802
Email	fxd93@psu.edu
Phone	8148651415
Submit Date	2023-02-22
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-04-03
Release Version	1

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Treatment:

Treatment ID:	TR002593
Treatment Summary:	Thawed on ice, serum sample was mixed with 4 volume extraction solvent of ice-cold methanol containing indole-3-acetic acid-d4 and kynurenic acid-d5. Mixture was vortexed and incubated at −20 °C for 30 min. Following centrifugation at 12,000 × g for 15 min at 4℃, supernatant was collected and subsequently evaporated to dryness (Thermo Scientific, Waltham, MA) and dissolved in 10% acetonitrile containing 1 µM chlorpropamide. After centrifugation at 12,000 × g for 15 min at 4℃, supernatants were transferred to autosampler vials for LC-MS analysis.

Treatment ID:

TR002593

Treatment Summary:

Thawed on ice, serum sample was mixed with 4 volume extraction solvent of ice-cold methanol containing indole-3-acetic acid-d4 and kynurenic acid-d5. Mixture was vortexed and incubated at −20 °C for 30 min. Following centrifugation at 12,000 × g for 15 min at 4℃, supernatant was collected and subsequently evaporated to dryness (Thermo Scientific, Waltham, MA) and dissolved in 10% acetonitrile containing 1 µM chlorpropamide. After centrifugation at 12,000 × g for 15 min at 4℃, supernatants were transferred to autosampler vials for LC-MS analysis.