Summary of Study ST003142

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001953. The data can be accessed directly via it's Project DOI: 10.21228/M88M77 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003142
Study Title	Effect of liver Acox1 knockout on serum lipidome in mice
Study Summary	The liver gene expression of the peroxisomal β-oxidation enzyme acyl-coenzyme A oxidase 1 (ACOX1), which catabolizes very long chain fatty acids (VLCFA), increases in the context of obesity. To check if liver peroxisomal fatty acids beta-oxidation deficiency will affect whole body metabolic homeostasis through circulating lipids. We analyzed serum samples from 5 WT and 5 Acox1-LKO mice.
Institute	Washington University in St. Louis
Last Name	Lu
First Name	Dongliang
Address	660 S. Euclid Ave.
Email	ludong-liang@wustl.edu
Phone	3147476766
Submit Date	2024-03-19
Analysis Type Detail	LC-MS
Release Date	2024-03-27
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003266
Sampleprep Summary:	The lipids extraction was performed strictly following the SOP established based on a modified Folch liquid-liquid extraction protocol. Briefly, an aliquot of 4.5 μL of each sample was vortexed with 1.5 μL of internal standard solution and methanol, followed by adding dichloromethane and vortexing for another 20 s. A clean-up step was performed with water and 10 seconds of vortex. Samples were allowed to equilibrate at room temperature for 10 min and centrifuged at 16,000 g for 10 min at 4°C. An aliquot of the organic layer was evaporated to dryness with a nitrogen blowdown evaporator. The residue was re-suspended in 4.5 μL of NovaMT MixB, vortexed for 1 min, and diluted with 40.5 μL of NovaMT MixA

Sampleprep ID:

SP003266

Sampleprep Summary:

The lipids extraction was performed strictly following the SOP established based on a modified Folch liquid-liquid extraction protocol. Briefly, an aliquot of 4.5 μL of each sample was vortexed with 1.5 μL of internal standard solution and methanol, followed by adding dichloromethane and vortexing for another 20 s. A clean-up step was performed with water and 10 seconds of vortex. Samples were allowed to equilibrate at room temperature for 10 min and centrifuged at 16,000 g for 10 min at 4°C. An aliquot of the organic layer was evaporated to dryness with a nitrogen blowdown evaporator. The residue was re-suspended in 4.5 μL of NovaMT MixB, vortexed for 1 min, and diluted with 40.5 μL of NovaMT MixA