Summary of Study ST001909
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001203. The data can be accessed directly via it's Project DOI: 10.21228/M86Q68 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001909 |
Study Title | ATF3 regulation of serine metabolism |
Study Type | stable isotope tracing |
Study Summary | ATF3 is a common stress sensor, and its expression can be induced by serine deprivation. The goal of this project is to determine whether ATF3 regulates serine biosynthesis. ATF3-wildtype and –knockout cells are cultured in complete or serine-free medium supplemented with 13C-6-glucose for 24 h for stable isotope tracing. The results show that ATF3 appears to promote serine biosynthesis. |
Institute | Augusta University |
Last Name | Yan |
First Name | Chunhong |
Address | 1410 Laney Walker Blvd, Augusta, GA, 30912, USA |
cyan@augusta.edu | |
Phone | 7067210099 |
Submit Date | 2021-08-11 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2021-10-02 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001999 |
Treatment Summary: | HCT116 cells (~50% confluence) were washed with PBS, and cultured in HEPES buffered Krebs-Ringer solution supplemented with 25 mM [U-13C]-D-glucose, 10% dialysed FBS, 2×MEM amino acids, 2× Vitamin Solution and 4 mM L-glutamine, with or without 0.4 mM serine and glycine, for 24 h. Cells were washed with PBS and metabolites extracted using 1 mL of acetonitrile:isopropanol:water (3:3:2, v/v/v) mixture. |