Summary of Study ST002841
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001779. The data can be accessed directly via it's Project DOI: 10.21228/M8RM7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002841 |
| Study Title | Collaborative role of YqgC and superoxide dismutase (MnSOD) in manganese intoxication: Replicate Experiment 2 |
| Study Type | Targeted MS Analysis |
| Study Summary | In this study, we are validating the mutual impact of yqgC and superoxide dismutase (MnSOD) deletion (YS strain) and resultant hypersensitivity to Manganese (Mn). Our genetic and physiological studies show that Mn intoxication is a result of distinct enzymatic vulnerabilities via alleviated expression of Mg-dependent, chorismate-utilizing enzymes of the menaquinone, siderophore, and tryptophan (MST) family (due to mismetalation). |
| Institute | Cornell University |
| Department | Medicine |
| Laboratory | Rhee Lab |
| Last Name | Soni |
| First Name | Vijay |
| Address | Weill Cornell Medical College, 413 E 69th St |
| vis2032@med.cornell.edu | |
| Phone | 9175391105 |
| Submit Date | 2023-08-28 |
| Num Groups | 10 |
| Total Subjects | 60 |
| Study Comments | NA |
| Publications | NA |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Chear Study | NA |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-09-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR002960 |
| Treatment Summary: | All strains were treated without (control group) or with (treated group) 0.15 mM of MnCl2 for 60 min at 37 ˚C. |
| Treatment Protocol ID: | NA |
| Treatment Protocol Filename: | NA |
| Treatment Protocol Comments: | NA |
| Treatment: | NA |
| Treatment Compound: | MnCl2 salt |
| Treatment Route: | Direct in bacterial culture |
| Treatment Dose: | 0.15 mM |
| Treatment Dosevolume: | NA |
| Treatment Doseduration: | NA |
| Treatment Vehicle: | Water |
| Cell Storage: | -80 |
| Cell Growth Container: | Flask |
| Cell Growth Config: | 200 rpm |
| Cell Growth Rate: | 40-60 min |
| Cell Inoc Proc: | NA |
| Cell Media: | LB Media |
| Cell Envir Cond: | NA |
| Cell Harvesting: | acetonitrile:methanol:water (40:40:20) |
| Cell Pct Confluence: | 0.4 OD600 |
| Cell Media Lastchanged: | NA |
