Summary of Study ST003091

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001920. The data can be accessed directly via it's Project DOI: 10.21228/M8JB09 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003091
Study Title	T cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia
Study Type	Untargeted metabolomics
Study Summary	We report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models.
Institute	Translational Genomics Research Institute
Department	Integrated Mass Spectrometry Shared Resources
Last Name	Pirrotte
First Name	Patrick
Address	445 N 5th St Phoenix, AZ 85004
Email	ppirrotte@tgen.org
Phone	6023438400
Submit Date	2024-01-26
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-03-01
Release Version	1

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Treatment:

Treatment ID:	TR003215
Treatment Summary:	CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.

Treatment ID:

TR003215

Treatment Summary:

CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.