Summary of study ST000405

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000317. The data can be accessed directly via it's Project DOI: 10.21228/M87G7J This work is supported by NIH grant, U2C- DK119886.

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Study IDST000405
Study TitleMetabolomic Profiling in Early Pregnancy using Pre-Diagnostic Sera from Women Who Developed Placental Abruption
Study TypeMetabolomics Biocrates Panel
Study SummaryPlacental abruption (PA) is an ischemic placental disorder that results from the premature separation of the placenta from the wall of the uterus before delivery of the fetus. This disorder is associated with pre-term delivery, fetal death, maternal hemorrhagic shock, and renal failure. Several physiologic disturbances, such as oxidative stress, carbohydrate/fatty acid metabolism, and mitochondrial dysfunction, have been associated with ischemic placental disorder as well as with placental abruption. This preliminary study proposes to identify metabolites associated with incident placental abruption using existing serum and clinical data from a previously studied cohort. Metabolomics analysis will be carried out on maternal serum (51 cases and 51 controls) collected in early pregnancy (early second trimester).
Institute
RTI International
DepartmentDiscovery Sciences
LaboratorySystems and Translational Sciences
Last NameBurgess
First NameJason
Address3040 Cornwallis Rd, RTP, NC, 27609, USA
Emailjpb@rti.org
Phone919-541-6700
Submit Date2016-05-27
Num Groups4
Total Subjects102
Num Females102
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-05-27
Release Version1
Jason Burgess Jason Burgess
https://dx.doi.org/10.21228/M87G7J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000317
Project DOI:doi: 10.21228/M87G7J
Project Title:Metabolomic Profiling in Early Pregnancy using Pre-Diagnostic Sera from Women Who Developed Placental Abruption
Project Type:Metabolomics
Project Summary:Placental abruption is an ischemic placental disorder that results from the premature separation of the placenta from the wall of the uterus before delivery of the fetus. This disorder is associated with pre-term delivery, fetal death, maternal hemorrhagic shock, and renal failure. Several physiologic disturbances, such as oxidative stress, carbohydrate/fatty acid metabolism, and mitochondrial dysfunction, have been associated with ischemic placental disorder as well as with placental abruption. This preliminary study proposes to identify metabolites associated with incident placental abruption using existing serum and clinical data from a previously studied cohort. Metabolomics analysis will be carried out on maternal serum (51 cases and 51 controls) collected in early pregnancy (early second trimester).
Institute:Harvard School of Public Health
Department:Swedish Medical Center
Last Name:Williams
First Name:Michelle
Address:677 Huntington Ave. Kresge Room 905A, Boston, MA 02446
Email:mawilliams@hsph.harvard.edu
Phone:(617) 432 - 6477

Subject:

Subject ID:SU000426
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:female
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group Vaginal Bleeding
SA019356Ctrl_44259No PA N
SA019357Ctrl_90649No PA N
SA019358Ctrl_90829No PA N
SA019359Ctrl_90776No PA N
SA019360Ctrl_90984No PA N
SA019361Ctrl_90876No PA N
SA019362Ctrl_90713No PA N
SA019363Ctrl_91161No PA N
SA019364Ctrl_91075No PA N
SA019365Ctrl_91170No PA N
SA019366Ctrl_90920No PA N
SA019367Ctrl_91147No PA N
SA019368Ctrl_90922No PA N
SA019369Ctrl_90405No PA N
SA019370Ctrl_90779No PA N
SA019371Ctrl_90967No PA N
SA019372Ctrl_90842No PA N
SA019373Ctrl_90567No PA N
SA019374Ctrl_90234No PA N
SA019375Ctrl_90668No PA N
SA019376Ctrl_90675No PA N
SA019377Ctrl_33105No PA N
SA019378Ctrl_90917No PA N
SA019379Ctrl_90535No PA N
SA019380Ctrl_90761No PA N
SA019381Ctrl_88188No PA N
SA019382Ctrl_90986No PA N
SA019383Ctrl_91195No PA N
SA019384Ctrl_91005No PA N
SA019385Ctrl_90963No PA N
SA019386Ctrl_90781No PA N
SA019387Ctrl_90699No PA N
SA019388Ctrl_90902No PA N
SA019389Ctrl_90929No PA N
SA019390Ctrl_90752No PA N
SA019391Ctrl_88038No PA N
SA019392Ctrl_90215No PA N
SA019393Ctrl_90966No PA N
SA019394Ctrl_90368No PA N
SA019395Ctrl_90380No PA N
SA019396Ctrl_91108No PA N
SA019397Ctrl_90199No PA N
SA019398Ctrl_90462No PA N
SA019399Ctrl_90239No PA N
SA019400Ctrl_90666No PA UNK
SA019401Ctrl_90797No PA UNK
SA019402Ctrl_91047No PA Y
SA019403Ctrl_90783No PA Y
SA019404Ctrl_90789No PA Y
SA019405Ctrl_91247No PA Y
SA019406Ctrl_91091No PA Y
SA019407Case_70055PA N
SA019408Case_70066PA N
SA019409Case_70035PA N
SA019410Case_70044PA N
SA019411Case_70019PA N
SA019412Case_70012PA N
SA019413Case_70017PA N
SA019414Case_70065PA N
SA019415Case_70036PA N
SA019416Case_70057PA N
SA019417Case_70064PA N
SA019418Case_70060PA N
SA019419Case_70061PA N
SA019420Case_70081PA N
SA019421Case_70056PA N
SA019422Case_70068PA N
SA019423Case_70027PA N
SA019424Case_70074PA N
SA019425Case_70053PA N
SA019426Case_70045PA N
SA019427Case_70028PA N
SA019428Case_70080PA N
SA019429Case_70013PA N
SA019430Case_70031PA N
SA019431Case_70023PA N
SA019432Case_70047PA N
SA019433Case_70052PA N
SA019434Case_70033PA N
SA019435Case_70058PA N
SA019436Case_70043PA N
SA019437Case_70079PA N
SA019438Case_70082PA UNK
SA019439Case_44220PA UNK
SA019440Case_70030PA Y
SA019441Case_70073PA Y
SA019442Case_70018PA Y
SA019443Case_70075PA Y
SA019444Case_70041PA Y
SA019445Case_70070PA Y
SA019446Case_70086PA Y
SA019447Case_70069PA Y
SA019448Case_70076PA Y
SA019449Case_70062PA Y
SA019450Case_70016PA Y
SA019451Case_70020PA Y
SA019452Case_88144PA Y
SA019453Case_70083PA Y
SA019454Case_70029PA Y
SA019455Case_70015PA Y
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Collection:

Collection ID:CO000420
Collection Summary:Serum samples collected in the second trimester from pregnant women
Sample Type:Blood
Blood Serum Or Plasma:serum

Treatment:

Treatment ID:TR000440
Treatment Summary:no treatment

Sample Preparation:

Sampleprep ID:SP000433
Sampleprep Summary:Sample Preparation Prior to Biocrates p180 Kit Plate Analysis: Serum samples were thawed on ice and pooled QC samples were prepared. Samples (study and pooled QC samples) were then diluted, vortexed, and transferred into properly labeled 2 mL Lo-Bind eppendorf tubes and dried on a lyophilizer overnight. The residue was reconstituted with 30 µL of PBS buffer. Samples were then vortexed and then centrifuged at room temperature and at 16,000 rcf for 4.0 minutes. All study samples and pooled QC samples were splitted into two AbsoluteIDQ p180 Kit-96 wells plates (Plate 1502 and 1503). Biocrates Plate Preparation: The Biocrates p180 kit-96 wells plates were prepared following the AbsoluteIDQ p180 Kit metabolomics procedure. Briefly, an internal standard mix was added to 95 of the 96 wells. Next, zero samples, QC standards and calibration standards were added to their corresponding wells. The study samples and pooled QC Samples were then added to the appropriate wells and dried for 30 minutes under nitrogen flow. The plate was derivatized using a 5% Phenylisothiocyanate (PITC) solution in (1:1:1) Ethanol:Pyridine:Water and, then, incubated for 20 minutes followed by a drying step under nitrogen flow. An extraction solvent (5 mM ammonium acetate in methanol) was added to all wells. The plate was then vortexed and filtrated by centrifugation. After centrifugation, 150 µL was removed and transferred to a second 96-well plate (LC-MS/MS plate). This second plate was diluted with 150 µL of HPLC grade water for a subsequent LC-MS/MS assay (analysis of amino acids and biogenic amines). All the wells in the original plate was diluted with 400 µL of flow injection analysis (FIA) Running Solvent for a FIA-MS analysis (lipids, acylcarnitines, and hexose analysis).

Combined analysis:

Analysis ID AN000645
Analysis type MS
Chromatography type Biocrates P-180
Chromatography system Agilent 1100
Column Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 4000 QTrap
Ion Mode UNSPECIFIED
Units area

Chromatography:

Chromatography ID:CH000469
Chromatography Summary:Biocrates P180 analysis combines a RP for positive mode MS , a flow injection for both pos and neg mode MS
Instrument Name:Agilent 1100
Column Name:Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um)
Column Pressure:30-120 bar for RP and 4-50 bar for FIA
Column Temperature:50 c fro RP and RT for FIA
Flow Rate:0.5 ml/min for RP and 0.2 ml/min for FIA
Sample Injection:10 ul
Solvent A:water with 0.2% formic acid
Solvent B:acetonitrile with 0.2% formic acid for RP, Methanol with Biocrates FIA Solvent
Chromatography Type:Biocrates P-180

MS:

MS ID:MS000577
Analysis ID:AN000645
Instrument Name:ABI Sciex API 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Biocrates combines a pos and negative mode for analysis
Ion Mode:UNSPECIFIED
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