Summary of study ST001110

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000741. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ2Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001110
Study TitleInvestigating link between metabolism and circadian rhythm in Drosophila melanogaster
Study SummaryWe are investigating the link between metabolism, circadian rhythm, and a high-fat diet with an emphasis on the role of metabolites that affect protein post translational modifications. We've hypothesized that protein modifications, which are critical to circadian clock functions, can be affected by different metabolic profiles, such as an excess or lack of fat in the diet, that ultimately regulate changes in circadian biology.
Institute
University of California, Davis
DepartmentCollege of Biological Sciences
Last NameContreras
First NameAdam
Address202 Life Sciences Building, Kleiber Hall Drive, Davis, CA, 95616
Emailajcon@ucdavis.edu
Phone512-393-4773
Submit Date2018-10-19
Total Subjects96
Raw Data AvailableYes
Raw Data File Type(s).cdf
Analysis Type DetailGC-MS
Release Date2019-01-22
Release Version1
Adam Contreras Adam Contreras
https://dx.doi.org/10.21228/M8WQ2Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000741
Project DOI:doi: 10.21228/M8WQ2Z
Project Title:Untargeted GC-MS Analysis in Drosophila melanogaster
Project Summary:Untargeted GC-MS Analysis in Drosophila melanogaster.
Institute:University of California, Davis
Department:Genome and Biomedical Sciences Facility
Laboratory:WCMC Metabolomics Core
Last Name:Fiehn
First Name:Oliver
Address:1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Email:ofiehn@ucdavis.edu
Phone:(530) 754-8258

Subject:

Subject ID:SU001155
Subject Type:Insect
Subject Species:Drosophila melanogaster
Taxonomy ID:7227

Factors:

Subject type: Insect; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id Organ Treatment
SA075645Bodies Zt10 - Rep2_NFDBody Tissue Cornmeal Drosphila Diet
SA075646Bodies Zt10 - Rep3_NFDBody Tissue Cornmeal Drosphila Diet
SA075647Bodies Zt10 - Rep5_NFDBody Tissue Cornmeal Drosphila Diet
SA075648Bodies Zt10 - Rep1_NFDBody Tissue Cornmeal Drosphila Diet
SA075649Bodies Zt10 - Rep4_NFDBody Tissue Cornmeal Drosphila Diet
SA075650Bodies Zt4 - Rep5_NFDBody Tissue Cornmeal Drosphila Diet
SA075651Bodies Zt4 - Rep2_NFDBody Tissue Cornmeal Drosphila Diet
SA075652Bodies Zt4 - Rep3_NFDBody Tissue Cornmeal Drosphila Diet
SA075653Bodies Zt4 - Rep4_NFDBody Tissue Cornmeal Drosphila Diet
SA075654Bodies Zt10 - Rep6_NFDBody Tissue Cornmeal Drosphila Diet
SA075655Bodies Zt4 - Rep6_NFDBody Tissue Cornmeal Drosphila Diet
SA075656Bodies Zt16 - Rep3_NFDBody Tissue Cornmeal Drosphila Diet
SA075657Bodies Zt22 - Rep3_NFDBody Tissue Cornmeal Drosphila Diet
SA075658Bodies Zt22 - Rep4_NFDBody Tissue Cornmeal Drosphila Diet
SA075659Bodies Zt22 - Rep5_NFDBody Tissue Cornmeal Drosphila Diet
SA075660Bodies Zt22 - Rep6_NFDBody Tissue Cornmeal Drosphila Diet
SA075661Bodies Zt22 - Rep2_NFDBody Tissue Cornmeal Drosphila Diet
SA075662Bodies Zt22 - Rep1_NFDBody Tissue Cornmeal Drosphila Diet
SA075663Bodies Zt4 - Rep1_NFDBody Tissue Cornmeal Drosphila Diet
SA075664Bodies Zt16 - Rep4_NFDBody Tissue Cornmeal Drosphila Diet
SA075665Bodies Zt16 - Rep5_NFDBody Tissue Cornmeal Drosphila Diet
SA075666Bodies Zt16 - Rep6_NFDBody Tissue Cornmeal Drosphila Diet
SA075667Bodies Zt16 - Rep2_NFDBody Tissue Cornmeal Drosphila Diet
SA075668Bodies Zt16 - Rep1_NFDBody Tissue Cornmeal Drosphila Diet
SA075669Bodies Zt16 - Rep4_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075670Bodies Zt16 - Rep5_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075671Bodies Zt16 - Rep3_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075672Bodies Zt16 - Rep2_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075673Bodies Zt16 - Rep1_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075674Bodies Zt16 - Rep6_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075675Bodies Zt22 - Rep1_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075676Bodies Zt22 - Rep5_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075677Bodies Zt22 - Rep4_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075678Bodies Zt22 - Rep3_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075679Bodies Zt22 - Rep2_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075680Bodies Zt10 - Rep6_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075681Bodies Zt4 - Rep1_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075682Bodies Zt4 - Rep5_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075683Bodies Zt4 - Rep4_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075684Bodies Zt4 - Rep3_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075685Bodies Zt4 - Rep2_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075686Bodies Zt4 - Rep6_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075687Bodies Zt10 - Rep1_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075688Bodies Zt10 - Rep5_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075689Bodies Zt10 - Rep4_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075690Bodies Zt10 - Rep3_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075691Bodies Zt10 - Rep2_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075692Bodies Zt22 - Rep6_HFDBody Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075693Heads Zt4 - Rep2_NFDHead Tissue Cornmeal Drosphila Diet
SA075694Heads Zt16 - Rep5_NFDHead Tissue Cornmeal Drosphila Diet
SA075695Heads Zt16 - Rep4_NFDHead Tissue Cornmeal Drosphila Diet
SA075696Heads Zt16 - Rep3_NFDHead Tissue Cornmeal Drosphila Diet
SA075697Heads Zt16 - Rep2_NFDHead Tissue Cornmeal Drosphila Diet
SA075698Heads Zt16 - Rep6_NFDHead Tissue Cornmeal Drosphila Diet
SA075699Heads Zt22 - Rep1_NFDHead Tissue Cornmeal Drosphila Diet
SA075700Heads Zt22 - Rep5_NFDHead Tissue Cornmeal Drosphila Diet
SA075701Heads Zt22 - Rep4_NFDHead Tissue Cornmeal Drosphila Diet
SA075702Heads Zt22 - Rep3_NFDHead Tissue Cornmeal Drosphila Diet
SA075703Heads Zt22 - Rep2_NFDHead Tissue Cornmeal Drosphila Diet
SA075704Heads Zt16 - Rep1_NFDHead Tissue Cornmeal Drosphila Diet
SA075705Heads Zt10 - Rep6_NFDHead Tissue Cornmeal Drosphila Diet
SA075706Heads Zt4 - Rep5_NFDHead Tissue Cornmeal Drosphila Diet
SA075707Heads Zt4 - Rep4_NFDHead Tissue Cornmeal Drosphila Diet
SA075708Heads Zt4 - Rep3_NFDHead Tissue Cornmeal Drosphila Diet
SA075709Heads Zt22 - Rep6_NFDHead Tissue Cornmeal Drosphila Diet
SA075710Heads Zt4 - Rep6_NFDHead Tissue Cornmeal Drosphila Diet
SA075711Heads Zt10 - Rep1_NFDHead Tissue Cornmeal Drosphila Diet
SA075712Heads Zt10 - Rep5_NFDHead Tissue Cornmeal Drosphila Diet
SA075713Heads Zt10 - Rep4_NFDHead Tissue Cornmeal Drosphila Diet
SA075714Heads Zt10 - Rep3_NFDHead Tissue Cornmeal Drosphila Diet
SA075715Heads Zt10 - Rep2_NFDHead Tissue Cornmeal Drosphila Diet
SA075716Heads Zt4 - Rep1_NFDHead Tissue Cornmeal Drosphila Diet
SA075717Heads Zt4 - Rep1_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075718Heads Zt10 - Rep2_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075719Heads Zt10 - Rep3_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075720Heads Zt10 - Rep4_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075721Heads Zt10 - Rep5_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075722Heads Zt10 - Rep1_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075723Heads Zt4 - Rep6_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075724Heads Zt4 - Rep2_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075725Heads Zt4 - Rep3_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075726Heads Zt4 - Rep4_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075727Heads Zt4 - Rep5_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075728Heads Zt22 - Rep6_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075729Heads Zt10 - Rep6_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075730Heads Zt22 - Rep2_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075731Heads Zt22 - Rep3_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075732Heads Zt22 - Rep4_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075733Heads Zt22 - Rep5_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075734Heads Zt16 - Rep1_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075735Heads Zt22 - Rep1_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075736Heads Zt16 - Rep2_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075737Heads Zt16 - Rep6_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075738Heads Zt16 - Rep3_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075739Heads Zt16 - Rep4_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075740Heads Zt16 - Rep5_HFDHead Tissue Cornmeal Drosphila Diet + 15% coconut Oil
SA075741Standard FoodN/A Cornmeal Drosphila Diet
SA075742High Fat FoodN/A Cornmeal Drosphila Diet + 15% coconut Oil
Showing results 1 to 98 of 98

Collection:

Collection ID:CO001149
Collection Summary:After each subject completed the treatment for the required time, the head of the fly was removed from the body over dry ice. Each portion of tissue was placed in a 1.5 mL tube and stored at -80°C until shipped to the WCMC for analysis.
Sample Type:Tissue
Volumeoramount Collected:Variable, depending on the size of the fly.
Storage Conditions:-80℃
Collection Vials:1.5 mL
Storage Vials:1.5 mL
Collection Tube Temp:Dry Ice, -78.5°C

Treatment:

Treatment ID:TR001169
Treatment Summary:48 flies were split into two treatment groups, with 24 flies in each group. Group 1 consisted of a High Fat Diet (HFD, Cornmeal diet + 15% coconut oil) and Group 2 consisted of a Non-Fat Diet (NFD, Cornmeal). Within each group, flies were placed in one of four smaller groups, depending on how long they would be on either the HFD or NFD. These groups are ZT4, ZT10, ZT16, ZT22. Each of these smaller groups consisted of 6 flies, to create 4 groups of 6 flies each in both of the diet treatments.

Sample Preparation:

Sampleprep ID:SP001162
Sampleprep Summary:Add 1 mL of 3:3:2 Acetonitrile:Isopropyl Alcohol:LC-MS Grade Water to each preweighed sample. Vortex each sample for 5 seconds. Shake at 4°C for 5 minutes using the Orbital Mixing Chilling/Heating Plate. Keep remaining samples on ice before shaking. Centrifuge samples for 2 minutes at 14000 rcf. Aliquot two 450 µL portions of the supernatant. Submit one to analysis and save one as backup at -20°C. To the submitted sample, dry in Labconco Centrivap. Submit to derivatization.
Sampleprep Protocol Filename:SOP_blood-GCTOF-11082012_332.pdf
Sampleprep Protocol Comments:Extraction solvent is prepared previously. Begin procedure at Sample Preparation under step 3.
Sample Derivatization:Add 10 µL of prepared MeOX solution to each sample. Shake for 1.5 hours at 30°C. Add 91 µL of prepared MSTFA + FAME solution to each sample. Shake for 30 minutes at 37°C.

Combined analysis:

Analysis ID AN001804
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus HT TOF
Ion Mode POSITIVE
Units Peak Height

Chromatography:

Chromatography ID:CH001271
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS001662
Analysis ID:AN001804
Instrument Name:Leco Pegasus HT TOF
Instrument Type:GC-TOF
MS Type:EI
Ion Mode:POSITIVE
Ion Source Temperature:250°C
Ionization Energy:-70 eV
Dataformat:.CDF
Resolution Setting:17 spectra s-1
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