Summary of study ST001253

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000840. The data can be accessed directly via it's Project DOI: 10.21228/M83Q36 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001253
Study TitlePhenotyping Mouse blood metabolites in day and night in type 2 diabetes
Study Typetime course in a 24hr period
Study SummaryThis experiment compares the metabolites in control db/m and diabetic db/db in day and night.
Institute
Indiana University School of Medicine
DepartmentOphthalmology
LaboratoryMaria Grant Laboratory
Last NameBeli
First NameEleni
Address97 Lisburn Road
Emaile.beli@qub.ac.uk
Phone5176144409
Submit Date2019-09-24
Num Groups4
Total Subjects15
Num Males15
PublicationsNutrients.2019. Beli E. Loss of diurnal oscillatory rhythms in gut microbiota correlates with changes in circulating metabolites in type 2 diabetic, db/db mice
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2019-10-11
Release Version1
Eleni Beli Eleni Beli
https://dx.doi.org/10.21228/M83Q36
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000840
Project DOI:doi: 10.21228/M83Q36
Project Title:Phenotyping blood metabolites in day and night in type 2 diabetes
Project Summary:Our hypothesis is that diabetes leads to differential regulation of circulating metabolites during the day and the night. We performed an untargeted global metabolomics study of plasma murine samples collected from 10-month old control (db/m) and type 2 diabetic (db/db) mice (n=3). Plasma was collected in EDTA tubes at a day (ZT5) and at a night (ZT17) timepoint and was frozen. Samples were shipped on dry ice and analyzed by Metabolon Inc with their untargeted global metabolomic platform and using UPLC- MS/MS to detect metabolites.
Institute:Indiana University School of Medicine
Department:Ophthalmology
Laboratory:Maria Grant Laboratory
Last Name:Beli
First Name:Eleni
Address:97 Lisburn Road
Email:e.beli@qub.ac.uk
Phone:2890976461
Funding Source:NIH- NIE
Publications:Nutrients.2019. Beli E. Loss of diurnal oscillatory rhythms in gut microbiota correlates with changes in circulating metabolites in type 2 diabetic, db/db mice

Subject:

Subject ID:SU001321
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:db/db
Age Or Age Range:10 months
Weight Or Weight Range:70-80gr
Gender:Male
Animal Animal Supplier:Jackson B6.BKS(D)-Leprdb/J (stock number:000697) homozygous db/db mice
Animal Housing:Indiana University LARC
Animal Light Cycle:12:12

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id diabetes time of day
SA0912026541db/db ZT17
SA0912032891db/db ZT17
SA0912042831db/db ZT17
SA0912056924db/db ZT5
SA0912065111db/db ZT5
SA0912076914db/db ZT5
SA0912086991db/db ZT5
SA0912097021db/m ZT17
SA0912107022db/m ZT17
SA0912117011db/m ZT17
SA0912127004db/m ZT5
SA0912137001db/m ZT5
SA0912147002db/m ZT5
SA0912157003db/m ZT5
SA0912167005db/m ZT5
Showing results 1 to 15 of 15

Collection:

Collection ID:CO001315
Collection Summary:Upon euthanasia, blood was collected via cardiac puncture in EDTA tubes and stored on ice until plasma isolation. Plasma isolation was done at 4oC for 20 min 800g. Plasma immediately was frozen in liquid ntirogen and stored at -80oC until sended to Metabolon Inc
Sample Type:Blood (plasma)
Collection Method:cardiac puncture
Collection Location:INDIANAPOLIS
Collection Duration:1hr
Volumeoramount Collected:500ul
Storage Conditions:-80℃
Collection Vials:EDTA vials
Storage Vials:0.5ml microcentrifuge tubes
Collection Tube Temp:ice

Treatment:

Treatment ID:TR001336
Treatment Summary:Animals were fed ad lib for 10 months with chow diet (2018SX, Harlan, 18% protein, 5% fat, 5% fiber). We obtained plasma samples at ZT5 and ZT17. Mice were held in Controlled-humidity, temperature and light environment facility at Indiana University, Indianapolis

Sample Preparation:

Sampleprep ID:SP001329
Sampleprep Summary:Frozen tissues were kept in 0.5 ml round-bottomed Eppendorf tubes at -80oC and send to Metabolon Inc
Sampleprep Protocol ID:Metabolon
Sampleprep Protocol Filename:Method
Processing Storage Conditions:On ice
Sample Resuspension:100ul

Combined analysis:

Analysis ID AN002079 AN002080 AN002081 AN002082
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase HILIC
Chromatography system Waters Acquity Waters Acquity Waters Acquity Waters Acquity
Column Waters Acquity BEH C18 (100 x 2mm, 1.7um) Waters Acquity BEH C18 (100 x 2mm, 1.7um) Waters Acquity BEH C18 (100 x 2mm, 1.7um) Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE POSITIVE NEGATIVE NEGATIVE
Units VolNormImp Area counts VolNormImp Area counts VolNormImp Area counts VolNormImp Area counts

Chromatography:

Chromatography ID:CH001516
Chromatography Summary:LC/MS Pos Early: Waters Acquity , Reversed phase, BEH C18 (100 X2mm, 1.7um), accidic, positive ion conditions
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2mm, 1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH001517
Chromatography Summary:LC/MS Pos Late: Waters Acquity , Reversed phase, BEH C18 (100 X2mm, 1.7um), acidic positive ion conditions
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2mm, 1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH001518
Chromatography Summary:LC/MS negative: Waters Acquity , Reversed phase, separate column BEH C18 (100 X2mm, 1.7um), basic negative ion optimized conditions
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2mm, 1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH001519
Chromatography Summary:LC/MS Polar: Instrument Waters Acquity , Reversed phase, Column: BEH Amide (150 X2mm, 1.7um),
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
Chromatography Type:HILIC

MS:

MS ID:MS001930
Analysis ID:AN002079
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Heated electrosplay ionization HESI-II
Ion Mode:POSITIVE
  
MS ID:MS001931
Analysis ID:AN002080
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Heated electrosplay ionization HESI-II
Ion Mode:POSITIVE
  
MS ID:MS001932
Analysis ID:AN002081
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Heated electrosplay ionization HESI-II
Ion Mode:NEGATIVE
  
MS ID:MS001933
Analysis ID:AN002082
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Heated electrosplay ionization HESI-II
Ion Mode:NEGATIVE
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