Summary of Study ST001253
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000840. The data can be accessed directly via it's Project DOI: 10.21228/M83Q36 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001253 |
Study Title | Phenotyping Mouse blood metabolites in day and night in type 2 diabetes |
Study Type | time course in a 24hr period |
Study Summary | This experiment compares the metabolites in control db/m and diabetic db/db in day and night. |
Institute | Indiana University School of Medicine |
Department | Ophthalmology |
Laboratory | Maria Grant Laboratory |
Last Name | Beli |
First Name | Eleni |
Address | 97 Lisburn Road |
e.beli@qub.ac.uk | |
Phone | 5176144409 |
Submit Date | 2019-09-24 |
Num Groups | 4 |
Total Subjects | 15 |
Num Males | 15 |
Publications | Nutrients.2019. Beli E. Loss of diurnal oscillatory rhythms in gut microbiota correlates with changes in circulating metabolites in type 2 diabetic, db/db mice |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2019-10-11 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000840 |
Project DOI: | doi: 10.21228/M83Q36 |
Project Title: | Phenotyping blood metabolites in day and night in type 2 diabetes |
Project Summary: | Our hypothesis is that diabetes leads to differential regulation of circulating metabolites during the day and the night. We performed an untargeted global metabolomics study of plasma murine samples collected from 10-month old control (db/m) and type 2 diabetic (db/db) mice (n=3). Plasma was collected in EDTA tubes at a day (ZT5) and at a night (ZT17) timepoint and was frozen. Samples were shipped on dry ice and analyzed by Metabolon Inc with their untargeted global metabolomic platform and using UPLC- MS/MS to detect metabolites. |
Institute: | Indiana University School of Medicine |
Department: | Ophthalmology |
Laboratory: | Maria Grant Laboratory |
Last Name: | Beli |
First Name: | Eleni |
Address: | 97 Lisburn Road |
Email: | e.beli@qub.ac.uk |
Phone: | 2890976461 |
Funding Source: | NIH- NIE |
Publications: | Nutrients.2019. Beli E. Loss of diurnal oscillatory rhythms in gut microbiota correlates with changes in circulating metabolites in type 2 diabetic, db/db mice |
Subject:
Subject ID: | SU001321 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | db/db |
Age Or Age Range: | 10 months |
Weight Or Weight Range: | 70-80gr |
Gender: | Male |
Animal Animal Supplier: | Jackson B6.BKS(D)-Leprdb/J (stock number:000697) homozygous db/db mice |
Animal Housing: | Indiana University LARC |
Animal Light Cycle: | 12:12 |
Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | diabetes | time of day |
---|---|---|---|
SA091202 | 6541 | db/db | ZT17 |
SA091203 | 2891 | db/db | ZT17 |
SA091204 | 2831 | db/db | ZT17 |
SA091205 | 6924 | db/db | ZT5 |
SA091206 | 5111 | db/db | ZT5 |
SA091207 | 6914 | db/db | ZT5 |
SA091208 | 6991 | db/db | ZT5 |
SA091209 | 7021 | db/m | ZT17 |
SA091210 | 7022 | db/m | ZT17 |
SA091211 | 7011 | db/m | ZT17 |
SA091212 | 7004 | db/m | ZT5 |
SA091213 | 7001 | db/m | ZT5 |
SA091214 | 7002 | db/m | ZT5 |
SA091215 | 7003 | db/m | ZT5 |
SA091216 | 7005 | db/m | ZT5 |
Showing results 1 to 15 of 15 |
Collection:
Collection ID: | CO001315 |
Collection Summary: | Upon euthanasia, blood was collected via cardiac puncture in EDTA tubes and stored on ice until plasma isolation. Plasma isolation was done at 4oC for 20 min 800g. Plasma immediately was frozen in liquid ntirogen and stored at -80oC until sended to Metabolon Inc |
Sample Type: | Blood (plasma) |
Collection Method: | cardiac puncture |
Collection Location: | INDIANAPOLIS |
Collection Duration: | 1hr |
Volumeoramount Collected: | 500ul |
Storage Conditions: | -80℃ |
Collection Vials: | EDTA vials |
Storage Vials: | 0.5ml microcentrifuge tubes |
Collection Tube Temp: | ice |
Treatment:
Treatment ID: | TR001336 |
Treatment Summary: | Animals were fed ad lib for 10 months with chow diet (2018SX, Harlan, 18% protein, 5% fat, 5% fiber). We obtained plasma samples at ZT5 and ZT17. Mice were held in Controlled-humidity, temperature and light environment facility at Indiana University, Indianapolis |
Sample Preparation:
Sampleprep ID: | SP001329 |
Sampleprep Summary: | Frozen tissues were kept in 0.5 ml round-bottomed Eppendorf tubes at -80oC and send to Metabolon Inc |
Sampleprep Protocol ID: | Metabolon |
Sampleprep Protocol Filename: | Method |
Processing Storage Conditions: | On ice |
Sample Resuspension: | 100ul |
Combined analysis:
Analysis ID | AN002079 | AN002080 | AN002081 | AN002082 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | HILIC |
Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
Units | VolNormImp Area counts | VolNormImp Area counts | VolNormImp Area counts | VolNormImp Area counts |
Chromatography:
Chromatography ID: | CH001516 |
Chromatography Summary: | LC/MS Pos Early: Waters Acquity , Reversed phase, BEH C18 (100 X2mm, 1.7um), accidic, positive ion conditions |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001517 |
Chromatography Summary: | LC/MS Pos Late: Waters Acquity , Reversed phase, BEH C18 (100 X2mm, 1.7um), acidic positive ion conditions |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001518 |
Chromatography Summary: | LC/MS negative: Waters Acquity , Reversed phase, separate column BEH C18 (100 X2mm, 1.7um), basic negative ion optimized conditions |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001519 |
Chromatography Summary: | LC/MS Polar: Instrument Waters Acquity , Reversed phase, Column: BEH Amide (150 X2mm, 1.7um), |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS001930 |
Analysis ID: | AN002079 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Heated electrosplay ionization HESI-II |
Ion Mode: | POSITIVE |
MS ID: | MS001931 |
Analysis ID: | AN002080 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Heated electrosplay ionization HESI-II |
Ion Mode: | POSITIVE |
MS ID: | MS001932 |
Analysis ID: | AN002081 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Heated electrosplay ionization HESI-II |
Ion Mode: | NEGATIVE |
MS ID: | MS001933 |
Analysis ID: | AN002082 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Heated electrosplay ionization HESI-II |
Ion Mode: | NEGATIVE |