Summary of study ST001523

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001005. The data can be accessed directly via it's Project DOI: 10.21228/M8SH6G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download all metabolite data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data (Contains raw data)
Study IDST001523
Study TitleThe effect of fasting and sirtuin overexpression on serum metabolome.
Study Typeuntargeted metabolomics
Study SummaryThe levels of the sirtuins SIRT1 and SIRT6 are regulated by nutrient availability in several mammalian tissues. In addition, overexpressing SIRT1 in mice improves healthspan, while SIRT6 overexpression increases both healthspan and lifespan. However, little is known about the impact of these sirtuins at the in vivo metabolomics level. Thus, we performed metabolomics on serum taken from 15 months old male mice overexpressing SIRT1, SIRT6 and SIRT1+SIRT6 as well as WT controls. Sera were obtained from mice at three nutritional time points - at fed state, after 4h morning fast and after 16h fast.
Institute
Bar Ilan University
DepartmentThe Mina & Everard Goodman Faculty of Life Sciences
LaboratoryLaboratory of Prof. Haim Cohen
Last NameCohen
First NameHaim
AddressBar Ilan University, Ramat Gan, Israel, 5290002
EmailHaim.Cohen@biu.ac.il
Phone+97235318383
Submit Date2020-09-10
Raw Data AvailableYes
Raw Data File Type(s).cdf
Analysis Type DetailGC-MS
Release Date2021-03-11
Release Version1
Haim Cohen Haim Cohen
https://dx.doi.org/10.21228/M8SH6G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001005
Project DOI:doi: 10.21228/M8SH6G
Project Title:The effect of fasting and sirtuin overexpression on serum metabolome.
Project Type:metabolomics
Project Summary:The levels of the sirtuins SIRT1 and SIRT6 are regulated by nutrient availability in several mammalian tissues. In addition, overexpressing SIRT1 in mice improves healthspan, while SIRT6 overexpression increases both healthspan and lifespan. However, little is known about the impact of these sirtuins at the in vivo metabolomics level. Thus, we performed metabolomics on serum taken from 15 months old male mice overexpressing SIRT1, SIRT6 and SIRT1+SIRT6 as well as WT controls. Sera were obtained from mice at three nutritional time points - at fed state, after 4h morning fast and after 16h fast.
Institute:Bar Ilan University
Department:The Mina & Everard Goodman Faculty of Life Sciences
Laboratory:Laboratory of Prof. Haim Cohen
Last Name:Haim
First Name:Cohen
Address:Bar Ilan University, Ramat Gan, Israel, 5290002
Email:Haim.Cohen@biu.ac.il
Phone:+97235318383
Funding Source:Intramural Research Program of the National Institute on Aging, NIH

Subject:

Subject ID:SU001597
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treamtent group
SA128376(20) 1+6 16h_072Sirt1+6 OE - 16h fast
SA128377(39) 1+6 16h_075Sirt1+6 OE - 16h fast
SA128378(16) 1+6 16h_071Sirt1+6 OE - 16h fast
SA128379(28) 1+6 16h_073Sirt1+6 OE - 16h fast
SA128380(36) 1+6 16h_074Sirt1+6 OE - 16h fast
SA128381(16) 1+6 4h_066Sirt1+6 OE - 4h fast
SA128382(20) 1+6 4h_067Sirt1+6 OE - 4h fast
SA128383(28) 1+6 4h_068Sirt1+6 OE - 4h fast
SA128384(39) 1+6 4h_070Sirt1+6 OE - 4h fast
SA128385(36) 1+6 4h_069Sirt1+6 OE - 4h fast
SA128386(20) 1+6 fed_062Sirt1+6 OE - fed
SA128387(28) 1+6 fed_063Sirt1+6 OE - fed
SA128388(16) 1+6 fed_061Sirt1+6 OE - fed
SA128389(36) 1+6 fed_064Sirt1+6 OE - fed
SA128390(39) 1+6 fed_065Sirt1+6 OE - fed
SA128355(21) 1 16h_039Sirt1 OE - 16h fast
SA128356(8) 1 16h_037Sirt1 OE - 16h fast
SA128357(35) 1 16h_040Sirt1 OE - 16h fast
SA128358(52) 1 16h_041Sirt1 OE - 16h fast
SA128359(55) 1 16h_042Sirt1 OE - 16h fast
SA128360(6) 1 16h_036Sirt1 OE - 16h fast
SA128361(17) 1 16h_038Sirt1 OE - 16h fast
SA128362(35) 1 4h_033Sirt1 OE - 4h fast
SA128363(17) 1 4h_031Sirt1 OE - 4h fast
SA128364(8) 1 4h_030Sirt1 OE - 4h fast
SA128365(6) 1 4h_029Sirt1 OE - 4h fast
SA128366(52) 1 4h_034Sirt1 OE - 4h fast
SA128367(21) 1 4h_032Sirt1 OE - 4h fast
SA128368(55) 1 4h_035Sirt1 OE - 4h fast
SA128369(55) 1 fed_028Sirt1 OE - fed
SA128370(6) 1 fed_022Sirt1 OE - fed
SA128371(17) 1 fed_024Sirt1 OE - fed
SA128372(21) 1 fed_025Sirt1 OE - fed
SA128373(35) 1 fed_026Sirt1 OE - fed
SA128374(52) 1 fed_027Sirt1 OE - fed
SA128375(8) 1 fed_023Sirt1 OE - fed
SA128391(14) 6 16h_056Sirt6 OE - 16h fast
SA128392(13) 6 16h_055Sirt6 OE - 16h fast
SA128393(19) 6 16h_058Sirt6 OE - 16h fast
SA128394(15) 6 16h_057Sirt6 OE - 16h fast
SA128395(29) 6 16h_059Sirt6 OE - 16h fast
SA128396(38) 6 16h_060Sirt6 OE - 16h fast
SA128397(19) 6 4h_052Sirt6 OE - 4h fast
SA128398(15) 6 4h_051Sirt6 OE - 4h fast
SA128399(38) 6 4h_054Sirt6 OE - 4h fast
SA128400(14) 6 4h_050Sirt6 OE - 4h fast
SA128401(29) 6 4h_053Sirt6 OE - 4h fast
SA128402(13) 6 4h_049Sirt6 OE - 4h fast
SA128403(15) 6 fed_045Sirt6 OE - fed
SA128404(14) 6 fed_044Sirt6 OE - fed
SA128405(19) 6 fed_046Sirt6 OE - fed
SA128406(13) 6 fed_043Sirt6 OE - fed
SA128407(29) 6 fed_047Sirt6 OE - fed
SA128408(38) 6 fed_048Sirt6 OE - fed
SA128409(18) WT 16h_017WT - 16h fast
SA128410(7) WT 16h_015WT - 16h fast
SA128411(65) WT 16h_021WT - 16h fast
SA128412(9) WT 16h_016WT - 16h fast
SA128413(48) WT 16h_019WT - 16h fast
SA128414(27) WT 16h_018WT - 16h fast
SA128415(56) WT 16h_020WT - 16h fast
SA128416(65) WT 4h_014WT - 4h fast
SA128417(48) WT 4h_012WT - 4h fast
SA128418(18) WT 4h_010WT - 4h fast
SA128419(9) WT 4h_009WT - 4h fast
SA128420(27) WT 4h_011WT - 4h fast
SA128421(56) WT 4h_013WT - 4h fast
SA128422(7) WT 4h_008WT - 4h fast
SA128423(9) WT fed_002WT - Fed
SA128424(18) WT fed_003WT - Fed
SA128425(65) WT fed_007WT - Fed
SA128426(7) WT fed_001WT - Fed
SA128427(56) WT fed_006WT - Fed
SA128428(48) WT fed_005WT - Fed
SA128429(27) WT fed_004WT - Fed
Showing results 1 to 75 of 75

Collection:

Collection ID:CO001592
Collection Summary:2 to 5 drops of blood were taken from the facial vein of each mouse using sterile 5mm goldenrod animal lancets. Blood was allowed to clot for 30-80 minutes at room temperature, then centrifuged 1,000 x g for 15 min at 4°C, snap frozen with liquid nitrogen and stored at -80°C.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001612
Treatment Summary:4 genotypes/3 time points, 5-7, Genotypes: WT, Sirt1-OE, Sirt6-OE, Sirt1+6-OE Time points: Fed - 4h fast- 16h fast

Sample Preparation:

Sampleprep ID:SP001605
Sampleprep Summary:2 to 5 drops of blood were taken from the facial vein of each mouse using sterile 5mm goldenrod animal lancets. Blood was allowed to clot for 30-80 minutes at room temperature, then centrifuged 1,000 x g for 15 min at 4°C, snap frozen with liquid nitrogen and stored at -80°C.

Combined analysis:

Analysis ID AN002541
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus IV GC
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type QTOF
MS instrument name Leco Pegasus IV TOF
Ion Mode POSITIVE
Units normalized peak height

Chromatography:

Chromatography ID:CH001861
Chromatography Summary:primary metabolism by GCTOF
Instrument Name:Leco Pegasus IV GC
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS002359
Analysis ID:AN002541
Instrument Name:Leco Pegasus IV TOF
Instrument Type:QTOF
MS Type:EI
MS Comments:A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da.
Ion Mode:POSITIVE
  logo