Summary of Study ST001851

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001168. The data can be accessed directly via it's Project DOI: 10.21228/M8QT2F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001851
Study Title	Quantitative analysis of bile acids in fecal samples from centenarians, elderly and young subjects.
Study Summary	Fecal samples from centenarians (>100 yo), elderly (85-89 yo) and young (21-55) subjects were analysed using LC-MS/MS. 48 bile acids were measured by targeted metabolomics.
Institute	Keio University School of Medicine
Department	Dept of Microbiology and Immunology
Last Name	Koji
First Name	Atarashi
Address	35 Shinanomachi, Shinjuku-ku, Tokyo, JAPAN
Email	kojiatarashi@keio.jp
Phone	0353633769
Submit Date	2021-05-24
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2021-07-07
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR001168
Project DOI:	doi: 10.21228/M8QT2F
Project Title:	Bile acid composition in Human fecal samples
Project Summary:	Centenarians, or individuals who have lived more than a century, represent the ultimate model of successful longevity associated with decreased susceptibility to ageing-associated illness and chronic inflammation. The gut microbiota is considered to be a critical determinant of human health and longevity. Here we show that centenarians (average 107 yo) have a distinct gut microbiome enriched in microbes capable of generating unique secondary bile acids, including iso-, 3-oxo-, allo-, 3-oxoallo-, and isoallo-lithocholic acid (LCA), as compared to elderly (85-89 yo) and young (21-55 yo) controls.
Institute:	Keio University
Department:	School of Medicine
Laboratory:	Dept of Microbiology and Immunology
Last Name:	Atarashi
First Name:	Koji
Address:	35 Shinanomachi, Shinjuku-ku, Tokyo, JAPAN
Email:	kojiatarashi@keio.jp
Phone:	0353633769

Subject:

Subject ID:	SU001928
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Category
SA172722	CE081	Centenarian
SA172723	CE095	Centenarian
SA172724	CE079	Centenarian
SA172725	CE134	Centenarian
SA172726	CE074	Centenarian
SA172727	CE065-2	Centenarian
SA172728	CE072	Centenarian
SA172729	CE150	Centenarian
SA172730	CE077	Centenarian
SA172731	CE162	Centenarian
SA172732	CE169	Centenarian
SA172733	CE170	Centenarian
SA172734	CE171	Centenarian
SA172735	CE167	Centenarian
SA172736	CE166	Centenarian
SA172737	CE029-2	Centenarian
SA172738	CE164	Centenarian
SA172739	CE165	Centenarian
SA172740	CE159	Centenarian
SA172741	CE028-2	Centenarian
SA172742	CE148	Centenarian
SA172743	CE149	Centenarian
SA172744	CE153	Centenarian
SA172745	CE145	Centenarian
SA172746	CE143	Centenarian
SA172747	CE138	Centenarian
SA172748	CE139	Centenarian
SA172749	CE140	Centenarian
SA172750	CE154	Centenarian
SA172751	CE155	Centenarian
SA172752	CE006	Centenarian
SA172753	CE007	Centenarian
SA172754	CE008	Centenarian
SA172755	CE004	Centenarian
SA172756	CE003-2	Centenarian
SA172757	CE157	Centenarian
SA172758	CE001	Centenarian
SA172759	CE173	Centenarian
SA172760	CE175	Centenarian
SA172761	CE088	Centenarian
SA172762	CE092	Centenarian
SA172763	CE100	Centenarian
SA172764	CE087-2	Centenarian
SA172765	CE087	Centenarian
SA172766	CE071	Centenarian
SA172767	CE073	Centenarian
SA172768	CE086	Centenarian
SA172769	CE101	Centenarian
SA172770	CE102	Centenarian
SA172771	CE029	Centenarian
SA172772	CE065	Centenarian
SA172773	CE030-2	Centenarian
SA172774	CE028	Centenarian
SA172775	CE003	Centenarian
SA172776	CE020	Centenarian
SA172777	CE024	Centenarian
SA172778	CE068	Centenarian
SA172779	CE062	Centenarian
SA172780	CE182	Centenarian
SA172781	CE183	Centenarian
SA172782	CE184	Centenarian
SA172783	CE180	Centenarian
SA172784	CE179	Centenarian
SA172785	CE176	Centenarian
SA172786	CE177	Centenarian
SA172787	CE178	Centenarian
SA172788	CE185	Centenarian
SA172789	CE188	Centenarian
SA172790	CE050	Centenarian
SA172791	CE055	Centenarian
SA172792	CE061	Centenarian
SA172793	CE046	Centenarian
SA172794	CE042	Centenarian
SA172795	CE030	Centenarian
SA172796	CE032	Centenarian
SA172797	CE137	Centenarian
SA172798	CE161	Centenarian
SA172799	CE096	Centenarian
SA172800	CE097	Centenarian
SA172801	CE098	Centenarian
SA172802	CE093	Centenarian
SA172803	CE092-2	Centenarian
SA172804	CE089	Centenarian
SA172805	CE090	Centenarian
SA172806	CE091	Centenarian
SA172807	CE099	Centenarian
SA172808	CE100-2	Centenarian
SA172809	CE106	Centenarian
SA172810	CE107	Centenarian
SA172811	CE108	Centenarian
SA172812	CE105	Centenarian
SA172813	CE104	Centenarian
SA172814	CE101-2	Centenarian
SA172815	CE102-2	Centenarian
SA172816	CE103	Centenarian
SA172817	CE088-2	Centenarian
SA172818	CE087-3	Centenarian
SA172819	CE055-2	Centenarian
SA172820	CE061-2	Centenarian
SA172821	CE062-2	Centenarian

Showing page 1 of 4 Results: 1 2 3 4 Next Showing results 1 to 100 of 387

Collection:

Collection ID:	CO001921
Collection Summary:	Stool samples were collected by the individuals using sterile faeces containers and transported to the lab in cooling-box with ice and Anaero pack within 24 hours of sample collection. Immediately after arriving, samples were aliquoted and stored at -80°C until further analysis.
Sample Type:	Feces
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR001940
Treatment Summary:	Frozen feces were crushed with a metal bead using a Multi-Beads Shocker (Yasui Kikai).

Sample Preparation:

Sampleprep ID:	SP001934
Sampleprep Summary:	Accurately weighed freeze-crushed faecal samples were resuspended in 20 times the volume of water (e.g. 12.5 mg of faece in 250 μL water). 250 μL of faecal suspension was homogenized in 747 μL of 0.27 N NaOH by ultrasonication for 1 hr in a screw-cap glass vial containing 3 μL of deuterium-labelled internal standards (d4-CA, d4-GCDCA, d4-TCDCA, d4-CDCA-3S, and d4-LCA, 50 μM each). After incubation for 1 hr at room temperature, pH was adjusted to 8.0 using 8 N HCl, mixed with 110 μL of 0.5 M EDTA/0.5 M TrisHCl. The solution was centrifuged at 15,000 rpm and the supernatant was transferred onto a solid-phase extraction cartridge (Agilent Bond Elut C18, 100 mg/3 mL, preconditioned with 1 mL of methanol and 3 mL of water, three times). The cartridge was washed with 1 mL of water and captured bile acids were eluted with 300 μL of 90% ethanol.

Sampleprep ID:

SP001934

Sampleprep Summary:

Accurately weighed freeze-crushed faecal samples were resuspended in 20 times the volume of water (e.g. 12.5 mg of faece in 250 μL water). 250 μL of faecal suspension was homogenized in 747 μL of 0.27 N NaOH by ultrasonication for 1 hr in a screw-cap glass vial containing 3 μL of deuterium-labelled internal standards (d4-CA, d4-GCDCA, d4-TCDCA, d4-CDCA-3S, and d4-LCA, 50 μM each). After incubation for 1 hr at room temperature, pH was adjusted to 8.0 using 8 N HCl, mixed with 110 μL of 0.5 M EDTA/0.5 M TrisHCl. The solution was centrifuged at 15,000 rpm and the supernatant was transferred onto a solid-phase extraction cartridge (Agilent Bond Elut C18, 100 mg/3 mL, preconditioned with 1 mL of methanol and 3 mL of water, three times). The cartridge was washed with 1 mL of water and captured bile acids were eluted with 300 μL of 90% ethanol.

Combined analysis:

Analysis ID	AN002999
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Exion LC
Column	InertSustain C18
MS Type	ESI
MS instrument type	Triple quadrupole
MS instrument name	ABI Sciex Triple Quad 6500+
Ion Mode	UNSPECIFIED
Units	nM

Chromatography:

Chromatography ID:	CH002224
Chromatography Summary:	2 μL was injected to LC/ESI-MS/MS (Triple Quad 6500+ tandem mass spectrometer, equipped with an ESI probe and Exion LC AD ultra-high pressure liquid chromatography system; SCIEX). A separation column, InertSustain C18 (150 mm × 2.1 mm ID, 3 μm particle size; GL Sciences Inc.), was utilized at 40 °C. A mixture of 10 mM ammonium acetate and acetonitrile was used as the eluent and the separation was carried out by linear gradient elution at a flow rate of 0.2 mL/min. The mobile phase composition was gradually changed as follows: ammonium acetate-acetonitrile (86:14, v/v) for 0.5 min, (78:22, v/v) for 0.5-5 min, (72:28, v/v) for 5-28 min, (46:54, v/v) for 28-55 min, (2:98, v/v) for 55-66 min, and (2:98, v/v) for 4 min. The total run time was 70 min.
Instrument Name:	Exion LC
Column Name:	InertSustain C18
Column Temperature:	40
Flow Gradient:	ammonium acetate-acetonitrile (86:14, v/v) for 0.5 min, (78:22, v/v) for 0.5-5 min, (72:28, v/v) for 5-28 min, (46:54, v/v) for 28-55 min, (2:98, v/v) for 55-66 min, and (2:98, v/v) for 4 min. The total run time was 70 min.
Flow Rate:	0.2 mL/min
Solvent A:	100% water; 10mM ammonium acetate
Solvent B:	100% acetonitrile
Chromatography Type:	Reversed phase

MS:

MS ID:	MS002788
Analysis ID:	AN002999
Instrument Name:	ABI Sciex Triple Quad 6500+
Instrument Type:	Triple quadrupole
MS Type:	ESI
MS Comments:	The following MS parameters were used for the positive ion MRM mode: ion spray voltage; 5,500 V, interface temperature; 500 °C, curtain gas; 30 psi, collision gas (nitrogen); 9 psi, nebulizing gas; 80 psi, and heater gas; 80 psi. For the negative ion MRM mode: ion spray voltage; -4,500 V, interface temperature; 500 °C, curtain gas; 30 psi, collision gas (nitrogen); 9 psi, nebulizing gas; 80 psi, and heater gas; 80 psi. Samples were obtained using Analyst software ver1.71 and analysed using SCIEX OS-MQ software ver1.7.0.36606.
Ion Mode:	UNSPECIFIED