Summary of Study ST002069

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001311. The data can be accessed directly via it's Project DOI: 10.21228/M88113 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002069
Study TitleSystemic impact of the expression of the mitochondrial alternative oxidase on Drosophila development
Study SummaryDespite the beneficial effects shown when the mitochondrial alternative oxidase AOX from Ciona intestinalis (Tunicata: Ascidiacea) is xenotopically expressed in mammalian and insect models, important detrimental outcomes have also been reported, raising concerns regarding its envisioned deployment as a therapy enzyme for human mitochondrial and related diseases. We have shown previously that AOX-expressing flies present a dramatic drop in pupal viability when the larvae are cultured on a low nutrient (LN) diet, indicating that AOX interferes with normal developmental metabolism. Here, we applied the metabolomics approach to gain insights into the molecular basis of the fatal developmental interaction between AOX expression and LN diet. We investigated the whole-body metabolome of AOX-expressing and control larvae cultured on SD and LN diets.
Tampere University
DepartmentFaculty of Medicine and Health Technology
Last NameDufour
First NameEric
AddressArvo Ylpön katu 34, 33520 Tampere, Finlândia
Phone+358 (0)50 413 0493
Submit Date2022-01-29
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-01-10
Release Version1
Eric Dufour Eric Dufour application/zip

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Project ID:PR001311
Project DOI:doi: 10.21228/M88113
Project Title:Molecular alterations caused by mitochondrial alternative oxidase (AOX) expression in Drosophila melanogaster development
Project Summary:Whole-body metabolome of AOX-expressing and control larvae of Drosophila melanogaster cultured on standard (SD) and low-nutrient (LN) diets
Institute:Tampere University
Department:Faculty of Medicine and Health Technology
Last Name:Dufour
First Name:Eric
Address:Arvo Ylpön katu 34, 33520 Tampere, Finlândia
Phone:+358 (0)50 413 0493
Funding Source:European Union (Marie Curie International Incoming Fellowship GA328988


Subject ID:SU002155
Subject Type:Insect
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Age Or Age Range:3rd instar larvae


Subject type: Insect; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id Genotype of fly lines Genotype Shorthand Diet Condition
SA194788SS-0172/2;daGAL4/daGAL4 C Low Nutrient (LN) LN
SA194789SS-0152/2;daGAL4/daGAL4 C Low Nutrient (LN) LN
SA194790SS-0182/2;daGAL4/daGAL4 C Low Nutrient (LN) LN
SA194791SS-0192/2;daGAL4/daGAL4 C Low Nutrient (LN) LN
SA194792SS-0142/2;daGAL4/daGAL4 C Low Nutrient (LN) LN
SA194793SS-0202/2;daGAL4/daGAL4 C Low Nutrient (LN) LN
SA194794SS-0162/2;daGAL4/daGAL4 C Low Nutrient (LN) LN
SA194795SS-0052/2;daGAL4/daGAL4 C Standard (SD) C
SA194796SS-0042/2;daGAL4/daGAL4 C Standard (SD) C
SA194797SS-0032/2;daGAL4/daGAL4 C Standard (SD) C
SA194798SS-0012/2;daGAL4/daGAL4 C Standard (SD) C
SA194799SS-0022/2;daGAL4/daGAL4 C Standard (SD) C
SA194785blank2- - - -
SA194786blank1- - - -
SA194787blank3- - - -
SA194800SS-025UAS-AOXwtF6/2; daGAL4/3 AOX Low Nutrient (LN) LN-AOX
SA194801SS-024UAS-AOXwtF6/2; daGAL4/3 AOX Low Nutrient (LN) LN-AOX
SA194802SS-021UAS-AOXwtF6/2; daGAL4/3 AOX Low Nutrient (LN) LN-AOX
SA194803SS-023UAS-AOXwtF6/2; daGAL4/3 AOX Low Nutrient (LN) LN-AOX
SA194804SS-010UAS-AOXwtF6/2; daGAL4/3 AOX Standard (SD) AOX
SA194805SS-009UAS-AOXwtF6/2; daGAL4/3 AOX Standard (SD) AOX
SA194806SS-007UAS-AOXwtF6/2; daGAL4/3 AOX Standard (SD) AOX
SA194807SS-011UAS-AOXwtF6/2; daGAL4/3 AOX Standard (SD) AOX
SA194808SS-008UAS-AOXwtF6/2; daGAL4/3 AOX Standard (SD) AOX
Showing results 1 to 24 of 24


Collection ID:CO002148
Collection Summary:Ten randomly selected, wandering L3 larvae (100-115 hrs post-egg laying in SD diet; 125-140 hrs in LN diet) per biological replica (5 in total) were collected, briefly rinsed in ultrapure water, and flash-frozen in liquid N2.
Sample Type:Larvae


Treatment ID:TR002167
Treatment Summary:The fly lines were maintained on a standard (SD) or a low-nutrient (LN) diet, with a 12/12h of light/dark photoperiod cycle at 25 °C. The eggs were allowed to develop until reaching the wandering L3 larval stage for collection. The LN diet consisted of 3.5% yeast extract, 1% agar, and antimicrobials (0.1% nipagin and 0.5% propionic acid).

Sample Preparation:

Sampleprep ID:SP002161
Sampleprep Summary:: Frozen larvae were homogenized in 1 ml cold (-80 °C) 80% methanol on dry ice using a probe homogenizer. Samples were clarified by centrifugation for 5 min at 16,000 x gmax at 4 °C and the supernatants were transferred to new Eppendorf tubes. The remaining pellets were resuspended in RIPA lysis buffer and total protein content was determined by the BCA method. A volume of the supernatant equivalent to 5 µg of protein was transferred to a glass vial, dried using an EZ-2 Elite evaporator (SP Scientific), and stored at -80 °C until analysis by LC-MS.

Combined analysis:

Analysis ID AN003378 AN003379
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system UltiMate 3000RSLC HPLC (Thermo Scientific) UltiMate 3000RSLC HPLC (Thermo Scientific)
Column Phenomenex Luna NH2 (150 x 2mm) Phenomenex Luna NH2 (150 x 2mm)
MS instrument type Ion trap Ion trap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Units intensity (area under the curve) intensity (area under the curve)


Chromatography ID:CH002497
Chromatography Summary:Separation was achieved using a linear gradient from 15% to 90% A over 18 min. This was followed by an isocratic step at 90% A for 9 min and re-equilibration to the initial 15% A for 7 min.
Instrument Name:UltiMate 3000RSLC HPLC (Thermo Scientific)
Column Name:Phenomenex Luna NH2 (150 x 2mm)
Column Temperature:27 C
Flow Rate:200 ul/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC


MS ID:MS003145
Analysis ID:AN003378
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Ion trap
MS Comments:MS1 scan range 65-975 m/z. MS/MS with inclusion list; target others when idle.
MS ID:MS003146
Analysis ID:AN003379
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Ion trap
MS Comments:MS1 scan range 65-975 m/z. MS/MS with inclusion list; target others when idle.