Summary of Study ST003091

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001920. The data can be accessed directly via it's Project DOI: 10.21228/M8JB09 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003091
Study TitleT cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia
Study TypeUntargeted metabolomics
Study SummaryWe report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models.
Institute
Translational Genomics Research Institute
DepartmentIntegrated Mass Spectrometry Shared Resources
Last NamePirrotte
First NamePatrick
Address445 N 5th St Phoenix, AZ 85004
Emailppirrotte@tgen.org
Phone6023438400
Submit Date2024-01-26
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-03-01
Release Version1
Patrick Pirrotte Patrick Pirrotte
https://dx.doi.org/10.21228/M8JB09
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001920
Project DOI:doi: 10.21228/M8JB09
Project Title:T cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia
Project Type:Untargeted metabolomics
Project Summary:We report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models.
Institute:Translational Genomics Research Institute
Department:Integrated Mass Spectrometry Shared Resources
Last Name:Pirrotte
First Name:Patrick
Address:445 N 5th St Phoenix, AZ 85004
Email:ppirrotte@tgen.org
Phone:6023438400
Contributors:Bin Zhang, Khyatiben V. Pathak, Brooke Lovell, Nate Hansen, Patrick Pirrote, Le Xuan Truong Nguyen, Guido Marcucci,

Subject:

Subject ID:SU003206
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA332985Norm. Area 088_0009_01B_R_TM48xWTxCMLxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F320)KO
SA332986Norm. Area 088_0009_01B_R_TM49xWTxCMLxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F321)KO
SA332987Norm. Area 088_0009_01B_R_TM50xWTxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F322)KO
SA332988Norm. Area 088_0009_01B_R_TM47xWTxCMLxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F319)KO
SA332989Norm. Area 088_0009_01B_R_TM45xKOxCMLxTAx24x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F317)KO
SA332990Norm. Area 088_0009_01B_R_TM43xKOxCMLxTAx24x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F315)KO
SA332991Norm. Area 088_0009_01B_R_TM44xKOxCMLxTAx24x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F316)KO
SA332992Norm. Area 088_0009_01B_R_TM51xWTxCMLxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F323)KO
SA332993Norm. Area 088_0009_01B_R_TM46xKOxCMLxTAx24x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F318)KO
SA332994Norm. Area 088_0009_01B_R_TM53xKOxCMLxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F325)KO
SA332995Norm. Area 088_0009_01D_R_TM21xWTxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F193)KO
SA332996Norm. Area 088_0009_01D_R_TM20xWTxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F192)KO
SA332997Norm. Area 088_0009_01D_R_TM19xWTxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F191)KO
SA332998Norm. Area 088_0009_01D_R_TM22xWTxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F194)KO
SA332999Norm. Area 088_0009_01B_R_TM56xKOxCMLxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F328)KO
SA333000Norm. Area 088_0009_01B_R_TM42xKOxCMLxTFx5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F314)KO
SA333001Norm. Area 088_0009_01B_R_TM54xKOxCMLxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F326)KO
SA333002Norm. Area 088_0009_01B_R_TM55xKOxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F327)KO
SA333003Norm. Area 088_0009_01B_R_TM52xKOxCMLxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F324)KO
SA333004Norm. Area 088_0009_01B_R_TM41xKOxCMLxTFx4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F313)KO
SA333005Norm. Area 088_0009_01C_R_TM51xWTxCMLxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F135)KO
SA333006Norm. Area 088_0009_01C_R_TM50xWTxCMLxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F134)KO
SA333007Norm. Area 088_0009_01C_R_TM49xWTxCMLxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F133)KO
SA333008Norm. Area 088_0009_01B_R_TM28xKOxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F300)KO
SA333009Norm. Area 088_0009_01B_R_TM27xKOxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F299)KO
SA333010Norm. Area 088_0009_01B_R_TM24xKOxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F296)KO
SA333011Norm. Area 088_0009_01B_R_TM25xKOxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F297)KO
SA333012Norm. Area 088_0009_01B_R_TM26xKOxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F298)KO
SA333013Norm. Area 088_0009_01D_R_TM28xKOxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F200)KO
SA333014Norm. Area 088_0009_01D_R_TM27xKOxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F199)KO
SA333015Norm. Area 088_0009_01B_R_TM38xKOxCMLxTFx1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F310)KO
SA333016Norm. Area 088_0009_01B_R_TM39xKOxCMLxTFx2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F311)KO
SA333017Norm. Area 088_0009_01B_R_TM40xKOxCMLxTFx3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F312)KO
SA333018Norm. Area 088_0009_01D_R_TM23xWTxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F195)KO
SA333019Norm. Area 088_0009_01D_R_TM24xKOxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F196)KO
SA333020Norm. Area 088_0009_01D_R_TM26xKOxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F198)KO
SA333021Norm. Area 088_0009_01D_R_TM25xKOxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F197)KO
SA333022Norm. Area 088_0009_01D_R_TM18xKOxTAx24x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F190)KO
SA333023Norm. Area 088_0009_01D_R_TM17xKOxTAx24x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F189)KO
SA333024Norm. Area 088_0009_01D_R_TM11xKOxTFx2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F183)KO
SA333025Norm. Area 088_0009_01D_R_TM10xKOxTFx1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F182)KO
SA333026Norm. Area 088_0009_01C_R_TM48xWTxCMLxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F132)KO
SA333027Norm. Area 088_0009_01D_R_TM12xKOxTFx3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F184)KO
SA333028Norm. Area 088_0009_01D_R_TM13xKOxTFx4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F185)KO
SA333029Norm. Area 088_0009_01C_R_TM26xKOxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F110)KO
SA333030Norm. Area 088_0009_01C_R_TM27xKOxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F111)KO
SA333031Norm. Area 088_0009_01C_R_TM28xKOxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F112)KO
SA333032Norm. Area 088_0009_01C_R_TM47xWTxCMLxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F131)KO
SA333033Norm. Area 088_0009_01C_R_TM46xKOxCMLxTAx24x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F130)KO
SA333034Norm. Area 088_0009_01C_R_TM40xKOxCMLxTFx3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F124)KO
SA333035Norm. Area 088_0009_01C_R_TM41xKOxCMLxTFx4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F125)KO
SA333036Norm. Area 088_0009_01C_R_TM43xKOxCMLxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F126)KO
SA333037Norm. Area 088_0009_01C_R_TM39xKOxCMLxTFx2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F123)KO
SA333038Norm. Area 088_0009_01C_R_TM38xKOxCMLxTFx1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F122)KO
SA333039Norm. Area 088_0009_01C_R_TM45xKOxCMLxTAx24x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F129)KO
SA333040Norm. Area 088_0009_01C_R_TM44xKOxCMLxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F128)KO
SA333041Norm. Area 088_0009_01C_R_TM25xKOxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F109)KO
SA333042Norm. Area 088_0009_01C_R_TM24xKOxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F108)KO
SA333043Norm. Area 088_0009_01C_R_TM12xKOxTFx3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F96)KO
SA333044Norm. Area 088_0009_01C_R_TM13xKOxTFx4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F97)KO
SA333045Norm. Area 088_0009_01C_R_TM14xKOxTFx5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F98)KO
SA333046Norm. Area 088_0009_01C_R_TM11xKOxTFx2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F95)KO
SA333047Norm. Area 088_0009_01C_R_TM10xKOxTFx1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F94)KO
SA333048Norm. Area 088_0009_01D_R_TM16xKOxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F188)KO
SA333049Norm. Area 088_0009_01D_R_TM15xKOxTAx24x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F187)KO
SA333050Norm. Area 088_0009_01D_R_TM14xKOxTFx5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F186)KO
SA333051Norm. Area 088_0009_01C_R_TM15xKOxTAx24x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F99)KO
SA333052Norm. Area 088_0009_01C_R_TM16xKOxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F100)KO
SA333053Norm. Area 088_0009_01C_R_TM21xWTxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F105)KO
SA333054Norm. Area 088_0009_01C_R_TM22xWTxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F106)KO
SA333055Norm. Area 088_0009_01C_R_TM23xWTxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F107)KO
SA333056Norm. Area 088_0009_01C_R_TM20xWTxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F104)KO
SA333057Norm. Area 088_0009_01C_R_TM19xWTxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F103)KO
SA333058Norm. Area 088_0009_01C_R_TM17xKOxTAx24x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F101)KO
SA333059Norm. Area 088_0009_01C_R_TM18xKOxTAx24x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F102)KO
SA333060Norm. Area 088_0009_01B_R_TM23xWTxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F295)KO
SA333061Norm. Area 088_0009_01B_R_TM21xWTxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F293)KO
SA333062Norm. Area 088_0009_01A_R_TM27xKOxTAx72x4xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F39)KO
SA333063Norm. Area 088_0009_01A_R_TM28xKOxTAx72x5xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F40)KO
SA333064Norm. Area 088_0009_01D_R_TM49xWTxCMLxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F222)KO
SA333065Norm. Area 088_0009_01A_R_TM26xKOxTAx72x3xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F38)KO
SA333066Norm. Area 088_0009_01A_R_TM25xKOxTAx72x2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F37)KO
SA333067Norm. Area 088_0009_01A_R_TM22xWTxTAx72x4xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F34)KO
SA333068Norm. Area 088_0009_01A_R_TM23xWTxTAx72x5xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F35)KO
SA333069Norm. Area 088_0009_01A_R_TM24xKOxTAx72x1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F36)KO
SA333070Norm. Area 088_0009_01D_R_TM48xWTxCMLxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F221)KO
SA333071Norm. Area 088_0009_01D_R_TM47xWTxCMLxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F220)KO
SA333072Norm. Area 088_0009_01D_R_TM41xKOxCMLxTFx4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F214)KO
SA333073Norm. Area 088_0009_01A_R_TM38xKOxCMLxTFx1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F49)KO
SA333074Norm. Area 088_0009_01A_R_TM39xKOxCMLxTFx2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F50)KO
SA333075Norm. Area 088_0009_01D_R_TM43xKOxCMLxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F215)KO
SA333076Norm. Area 088_0009_01D_R_TM43xKOxCMLxTFx5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F216)KO
SA333077Norm. Area 088_0009_01D_R_TM46xKOxCMLxTAx24x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F219)KO
SA333078Norm. Area 088_0009_01D_R_TM45xKOxCMLxTAx24x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F218)KO
SA333079Norm. Area 088_0009_01D_R_TM44xKOxCMLxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F217)KO
SA333080Norm. Area 088_0009_01A_R_TM21xWTxTAx72x3xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F33)KO
SA333081Norm. Area 088_0009_01A_R_TM20xWTxTAx72x2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F32)KO
SA333082Norm. Area 088_0009_01D_R_TM51xWTxCMLxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F224)KO
SA333083Norm. Area 088_0009_01D_R_TM50xWTxCMLxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F223)KO
SA333084Norm. Area 088_0009_01A_R_TM10xKOxTFx1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F22)KO
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Collection:

Collection ID:CO003199
Collection Summary:Tcells from miR142 WT and KO BCR ABL mice were isolated and subjected to resting or 24h activation.
Sample Type:T-cells
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003215
Treatment Summary:CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.

Sample Preparation:

Sampleprep ID:SP003212
Sampleprep Summary:Metabolite extraction on T cells were performed using 70% acetonitrile/methanol and 30% water. The metabolite extracts were subjected to untargeted metabolomics using HILIC and reversed phase chromatographies in positive and negative modes.
Processing Storage Conditions:4℃
Extraction Method:Polar solvent extraction
Extract Storage:-80℃
Sample Derivatization:none

Chromatography:

Chromatography ID:CH003819
Chromatography Summary:Reversed phase chromatography
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Thermo Hypersil Gold C18 (150x 2.1mm, 3uM)
Column Temperature:45
Flow Gradient:0%A for 1 min, 50% B for 4 min, 98%B for 3 min, 0% A for 5min
Flow Rate:0.3ml/min
Injection Temperature:4
Solvent A:Water, 0.1% formic acid
Solvent B:Acetonitrile, 0.1% formic acid
Transferline Temperature:275
Target Sample Temperature:4
Chromatography Type:Reversed phase
  
Chromatography ID:CH003820
Chromatography Summary:HILIC chromatography
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:99%B for 1min, 85%B for 2min, 75%B for 3 min, 30%B for 3 min, 99%B for 5 min
Flow Rate:0.35ml/min
Injection Temperature:4
Solvent A:10mM ammonium acetate, 95% Water, 5% Acetonitrile, 0.1% acetic acid
Solvent B:10mM ammonium acetate, 95% Acetonitrile, 95% Water, 0.1% acetic acid
Transferline Temperature:275
Target Sample Temperature:4
Chromatography Type:HILIC

Analysis:

Analysis ID:AN005056
Analysis Type:MS
Chromatography ID:CH003819
Has Rt:1
Rt Units:Minutes
Results File:ST003091_AN005056_Results.txt
Units:Peak area
  
Analysis ID:AN005057
Analysis Type:MS
Chromatography ID:CH003819
Has Rt:1
Rt Units:Minutes
Results File:ST003091_AN005057_Results.txt
Units:Peak area
  
Analysis ID:AN005058
Analysis Type:MS
Chromatography ID:CH003820
Has Rt:1
Rt Units:Minutes
Results File:ST003091_AN005058_Results.txt
Units:Peak area
  
Analysis ID:AN005059
Analysis Type:MS
Chromatography ID:CH003820
Has Rt:1
Rt Units:Minutes
Results File:ST003091_AN005059_Results.txt
Units:Peak area
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