Summary of Study ST003046
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001896. The data can be accessed directly via it's Project DOI: 10.21228/M8N72Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003046 |
| Study Title | Multi-Omics Plasma Signatures of Severe Injury with Influence of REBOA Intervention in a Swine Model. |
| Study Type | Original Research |
| Study Summary | Swine experienced controlled severe injury involving combinations of trauma, hemorrhagic shock, and disseminated complex blast injury (DCBI). After a period of hemorrhagic shock, resuscitation was performed using Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA) and shed blood transfusion. Blood samples were collected from sham and experiment swine. Blood samples for experiment swine were collected at baseline and periodically through the monitored time course. The plasma fraction were then subjected to mass spectrometry based metabolomics. Results indicated intervention using REBOA following polytrauma in our swine model induced distinct multi-omics alterations as a function of placement location. Namely, aortic balloon placement in Zone-1 worsened ischemic damage and mitochondrial dysfunction, patterns that continued through the monitored time course. |
| Institute | University of Colorado Anschutz Medical Campus |
| Laboratory | The D'Alessandro Lab |
| Last Name | LaCroix |
| First Name | Ian |
| Address | RC1-S L18-9403G E 17th Ave. Aurora, CO 80045 |
| ian.lacroix@cuanschutz.edu | |
| Phone | (303) 724-0096 |
| Submit Date | 2024-01-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-02-08 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003154 |
| Collection Summary: | Healthy adolescent, male Yorkshire swine (average weight 50.0 ± 2.7 kg) were acclimated in the animal facilities for a minimum of 3 days and examined by a veterinarian on the day of experimentation. In brief, on the day of surgery, swine anesthesia was induced via intramuscular injection of ketamine (20.0 mg/kg), xylazine (2.0 mg/kg), and acepromazine (0.2 mg/kg) followed by intubation under direct laryngoscopy. Continuous infusion anesthesia was maintained through a superficial ear vein using propofol (3 mg/kg/h) and fentanyl (3 mcg/kg/h). Lactated Ringer’s solution was administered at a rate of 10 mL/kg/h to overcome insensible losses. Mahurkar catheters (12 Fr) were placed in the femoral arteries and veins for vascular access. |
| Collection Protocol ID: | Animal Care and Use Committee approved this animal study under protocol #1050 |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
