Summary of Study ST001673

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001076. The data can be accessed directly via it's Project DOI: 10.21228/M8MH5J This work is supported by NIH grant, U2C- DK119886.

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Study IDST001673
Study TitleTargeted Sphingolipid analysis of HeLa silenced for or overexpressing GOLPH3 or LCS
Study SummaryA group of sequentially-acting enzymes operating at the branchpoint among sphingolipid synthetic pathways binds the Golgi-localised oncoprotein GOLPH3. GOLPH3 sorts these enzymes into vesicles for intra-Golgi retro-transport, acting as a component of the cisternae inter-conversion mechanisms. Through these effects, GOLPH3 controls the sub-Golgi localisation, and the lysosomal degradation rate of specific enzymes. Here we evaluated the impact of overexpressing or silencing GOLPH3 or its client enzyme lactosylceramide synthase (LCS) on the sphingolipid composition of HeLa cells by targeted lipid analysis.
Institute
École polytechnique fédérale de Lausanne (EPFL)
DepartmentIBI
LaboratoryUPDANGELO
Last NameD'Angelo
First NameGiovanni
AddressStation 15, Lausanne, Vaud, 1015, Switzerland
Emailgiovanni.dangelo@epfl.ch
Phone+41 216934276
Submit Date2021-01-29
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-02-26
Release Version1
Giovanni D'Angelo Giovanni D'Angelo
https://dx.doi.org/10.21228/M8MH5J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA154464GOLPH3_OE_1GOLPH3 overexpression
SA154465GOLPH3_OE_3GOLPH3 overexpression
SA154466GOLPH3_OE_2GOLPH3 overexpression
SA154467GOLPH3_KD_3GOLPH3 silencing
SA154468GOLPH3_KD_1GOLPH3 silencing
SA154469GOLPH3_KD_2GOLPH3 silencing
SA154470LCS_OE_2LCS overexpression
SA154471LCS_OE_1LCS overexpression
SA154472LCS_OE_3LCS overexpression
SA154473LCS_KD_3LCS silencing
SA154474LCS_KD_2LCS silencing
SA154475LCS_KD_1LCS silencing
SA154476CTRL_2no treatment
SA154477CTRL_1no treatment
SA154478CTRL_3no treatment
Showing results 1 to 15 of 15
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