Summary of Study ST001673
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001076. The data can be accessed directly via it's Project DOI: 10.21228/M8MH5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001673 |
Study Title | Targeted Sphingolipid analysis of HeLa silenced for or overexpressing GOLPH3 or LCS |
Study Summary | A group of sequentially-acting enzymes operating at the branchpoint among sphingolipid synthetic pathways binds the Golgi-localised oncoprotein GOLPH3. GOLPH3 sorts these enzymes into vesicles for intra-Golgi retro-transport, acting as a component of the cisternae inter-conversion mechanisms. Through these effects, GOLPH3 controls the sub-Golgi localisation, and the lysosomal degradation rate of specific enzymes. Here we evaluated the impact of overexpressing or silencing GOLPH3 or its client enzyme lactosylceramide synthase (LCS) on the sphingolipid composition of HeLa cells by targeted lipid analysis. |
Institute | École polytechnique fédérale de Lausanne (EPFL) |
Department | IBI |
Laboratory | UPDANGELO |
Last Name | D'Angelo |
First Name | Giovanni |
Address | Station 15, Lausanne, Vaud, 1015, Switzerland |
giovanni.dangelo@epfl.ch | |
Phone | +41 216934276 |
Submit Date | 2021-01-29 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-02-26 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001756 |
Sampleprep Summary: | Samples were fortified with an Internal standard Mix and lipids were extracted twice with an extraction mix consisting of 85:15 Ethyl acetate 70% Isopropanol. All lipids that were used in the Internal standard mix and in the Calibration mixes were purchased from Avanti Polar Lipids Inc. After evaporating the cell extract to dryness, the samples were reconstituted in the mobile phase. |