Summary of Study ST001214

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000811. The data can be accessed directly via it's Project DOI: 10.21228/M8VD62 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001214
Study TitleLipidommics in the serum of human subjects
Study SummaryWe aimed to determine the levels of the cold-induced 12-LOX products in patients with different degrees of body mass index (BMI). This analysis allowed us to infer about the role of these oxylipins in the pathophysiology of obesity.
Institute
Joslin Diabetes Center
Last NameLeiria
First NameLuiz
AddressOne Joslin Place, 02215, Boston-MA - USA
Emailluiz.leiria@joslin.harvard.edu
Phone617-309-1967
Submit Date2019-07-10
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Luiz Leiria Luiz Leiria
https://dx.doi.org/10.21228/M8VD62
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002025
Analysis type MS
Chromatography type Reversed phase
Chromatography system Ekspert MicroLC 200 system
Column Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um)
MS Type ESI
MS instrument type QTOF
MS instrument name ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE
Units Peak Area

MS:

MS ID:MS001878
Analysis ID:AN002025
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MS analysis was performed on a SCIEX TripleTOF® 5600+ system using the HR-MRM strategy consisting of a TOF MS experiment looped with multiple MS/MS experiments. MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Fullscan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX).
Ion Mode:NEGATIVE
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