Summary of Study ST001207
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000811. The data can be accessed directly via it's Project DOI: 10.21228/M8VD62 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001207 |
| Study Title | Lipidomics in the serum of cold exposed mice treated with 12-LOX inhibitor LOXBlock-1 |
| Study Summary | We aimed to investigate whether the cold-induced release of 12-LOX products into the circulation were dependent on 12-LOX activation. We pre-treated C57BL6/J mice with the pharmacological inhibitor LOXBlock-1 or its vehicle (DMSO), and after 15 minutes we placed them under cold temperature (5C) for 4 hours. A control group was injected with DMSO and kept at room temperature for the same 4 hours. After this period of time we, collected the blood, and obtained the serum fraction that was immediately frozen and submitted for untargeted lipidomics. |
| Institute | Joslin Diabetes Center |
| Last Name | Leiria |
| First Name | Luiz |
| Address | One Joslin Place, Boston-MA, 02215 |
| luiz.leiria@joslin.harvard.edu | |
| Phone | 617-309-1967 |
| Submit Date | 2019-06-26 |
| Num Groups | 3 |
| Study Comments | Joslin Diabetes Center affiliate of Harvard Medical School |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-07-17 |
| Release Version | 1 |
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Project:
| Project ID: | PR000811 |
| Project DOI: | doi: 10.21228/M8VD62 |
| Project Title: | 12-LOX metabolites in cold adaptation |
| Project Summary: | The goal of this project is to understand the role of the enzyme 12-lipoxygenase in the adaptive thermogenesis. We found this enzyme is activated by cold stimulation, then producing lipid metabolites in adipose tissue and releasing them into the circulation to regulate fuel utilisation and thermogenic pathways required for the cold adaptation. |
| Institute: | Joslin Diabetes Center |
| Department: | Integrative Physiology and Metabolism |
| Laboratory: | Yu-Hua Tseng lab |
| Last Name: | Leiria |
| First Name: | Luiz |
| Address: | One Joslin Place, Boston, MA-USA, 02215 |
| Email: | luiz.leiri@joslin.harvard.edu |
| Phone: | 1 6173091967 |
