Summary of Study ST000260

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000211. The data can be accessed directly via it's Project DOI: 10.21228/M84598 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000260
Study Title	Analysis of DJ-1 Knockout Mouse Brains
Study Type	Mouse genotype comparison
Study Summary	We analyzed metabolites in the brains of wild type mice and DJ-1 knockout mice. The DJ-1 knockout mouse is a model of an inherited form of Parkinson's disease.
Institute	National Institute on Aging
Department	Laboratory of Neurogenetics
Laboratory	Cell Biology and Gene Expression Section
Last Name	Hauser
First Name	David
Address	35 Lincoln Drive, BLDG 35, Room 1A-1012, Bethesda, MD 20892
Email	hauserd@mail.nih.gov
Phone	301-435-8995
Submit Date	2015-09-17
Num Groups	2
Total Subjects	25
Raw Data Available	No
Analysis Type Detail	GC-MS/LC-MS
Release Date	2017-10-03
Release Version	1

Select appropriate tab below to view additional metadata details:

Treatment:

Treatment ID:	TR000294
Treatment Summary:	The animals were either wild type of homozygous DJ-1 knockout animals. At 4 months of age, the mice were euthanized and their brains were processed for metabolomic analysis.
Animal Vet Treatments:	None
Animal Fasting:	No dietary interventions
Animal Endp Euthanasia:	The mice were euthanized with CO2 followed by cervical dislocation.
Animal Endp Tissue Coll List:	Cerebral Hemispheres
Animal Endp Tissue Proc Method:	Immediately after euthanization, the brains were removed and dissected on an ice cold stage. The cerebellum was removed and the two cerebral hemispheres were bisected along the longtiduinal fissue. The cerebral hemispheres were immediately frozen on dry ice in individual tubes. The frozen tissue was then sent to Metabolon for subsequent processing.