Summary of Study ST003046

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001896. The data can be accessed directly via it's Project DOI: 10.21228/M8N72Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003046
Study TitleMulti-Omics Plasma Signatures of Severe Injury with Influence of REBOA Intervention in a Swine Model.
Study TypeOriginal Research
Study SummarySwine experienced controlled severe injury involving combinations of trauma, hemorrhagic shock, and disseminated complex blast injury (DCBI). After a period of hemorrhagic shock, resuscitation was performed using Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA) and shed blood transfusion. Blood samples were collected from sham and experiment swine. Blood samples for experiment swine were collected at baseline and periodically through the monitored time course. The plasma fraction were then subjected to mass spectrometry based metabolomics. Results indicated intervention using REBOA following polytrauma in our swine model induced distinct multi-omics alterations as a function of placement location. Namely, aortic balloon placement in Zone-1 worsened ischemic damage and mitochondrial dysfunction, patterns that continued through the monitored time course.
Institute
University of Colorado Anschutz Medical Campus
LaboratoryThe D'Alessandro Lab
Last NameLaCroix
First NameIan
AddressRC1-S L18-9403G E 17th Ave. Aurora, CO 80045
Emailian.lacroix@cuanschutz.edu
Phone(303) 724-0096
Submit Date2024-01-17
Raw Data AvailableYes
Raw Data File Type(s)mzXML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-02-08
Release Version1
Ian LaCroix Ian LaCroix
https://dx.doi.org/10.21228/M8N72Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001896
Project DOI:doi: 10.21228/M8N72Z
Project Title:Multi-Omics Plasma Signatures of Severe Injury with Influence of REBOA Intervention in a Swine Model.
Project Type:Origninal Research
Project Summary:Swine experienced controlled severe injury involving combinations of trauma, hemorrhagic shock, and disseminated complex blast injury (DCBI). After a period of hemorrhagic shock, resuscitation was performed using REBOA and shed blood transfusion. Blood samples were collected from sham and experiment swine. Blood samples for experiment swine were collected at baseline and periodically through the monitored time course. The plasma fraction were then subjected to mass spectrometry based metabolomics.
Institute:1) Department of Biochemistry and Molecular Genetics, University of Colorado Denver – Anschutz Medical Campus, Aurora, CO, USA
Laboratory:The D'Alessandro Lab
Last Name:LaCroix
First Name:Ian
Address:12801 E 17th Ave, Aurora, Colorado, 80045, USA
Email:ian.lacroix@cuanschutz.edu
Phone:(303) 724-0096
Publications:DOI: 10.1097/SLA.0000000000005944

Subject:

Subject ID:SU003161
Subject Type:Mammal
Subject Species:Sus scrofa
Taxonomy ID:9823
Gender:Male

Factors:

Subject type: Mammal; Subject species: Sus scrofa (Factor headings shown in green)

mb_sample_id local_sample_id Pig_Model Time_from_End_of_Shock_min
SA33052294DCBI 0
SA330523152DCBI 0
SA330524108DCBI 0
SA330525145DCBI 0
SA33052678DCBI 0
SA330527131DCBI 0
SA330528134DCBI 120
SA33052997DCBI 120
SA33053081DCBI 120
SA330531155DCBI 120
SA330532111DCBI 120
SA33053390DCBI 120
SA330534148DCBI 120
SA330514130DCBI -120
SA330515144DCBI -120
SA330516151DCBI -120
SA33051777DCBI -120
SA330518107DCBI -120
SA330519128DCBI -120
SA33052086DCBI -120
SA33052193DCBI -120
SA33053591DCBI 180
SA330536135DCBI 180
SA33053782DCBI 180
SA330538156DCBI 180
SA330539149DCBI 180
SA330540112DCBI 180
SA33054198DCBI 180
SA330542113DCBI 240
SA330543157DCBI 240
SA330544150DCBI 240
SA33054583DCBI 240
SA33054699DCBI 240
SA330547136DCBI 240
SA33054892DCBI 240
SA330549146DCBI 30
SA330550132DCBI 30
SA330551153DCBI 30
SA33055279DCBI 30
SA330553109DCBI 30
SA33055495DCBI 30
SA33055588DCBI 30
SA33055680DCBI 60
SA33055796DCBI 60
SA33055889DCBI 60
SA330559110DCBI 60
SA330560133DCBI 60
SA330561154DCBI 60
SA330562147DCBI 60
SA33056370DCBI NA
SA33056471DCBI NA
SA33056569DCBI NA
SA3305699HS 0
SA3305702HS 0
SA33057116HS 0
SA33057212HS 120
SA33057319HS 120
SA3305745HS 120
SA3305668HS -120
SA3305671HS -120
SA33056815HS -120
SA33057513HS 180
SA3305766HS 180
SA33057720HS 180
SA33057821HS 240
SA33057914HS 240
SA3305807HS 240
SA33058117HS 30
SA33058210HS 30
SA3305833HS 30
SA3305844HS 60
SA33058511HS 60
SA33058618HS 60
SA330593163REBOA Zone I + DCBI 0
SA330594201REBOA Zone I + DCBI 0
SA330595138REBOA Zone I + DCBI 0
SA330596159REBOA Zone I + DCBI 0
SA330597187REBOA Zone I + DCBI 0
SA330598125REBOA Zone I + DCBI 120
SA330599166REBOA Zone I + DCBI 120
SA330600141REBOA Zone I + DCBI 120
SA330601204REBOA Zone I + DCBI 120
SA330602190REBOA Zone I + DCBI 120
SA330587121REBOA Zone I + DCBI -120
SA330588200REBOA Zone I + DCBI -120
SA330589137REBOA Zone I + DCBI -120
SA330590186REBOA Zone I + DCBI -120
SA330591162REBOA Zone I + DCBI -120
SA330592158REBOA Zone I + DCBI -120
SA330603142REBOA Zone I + DCBI 180
SA330604191REBOA Zone I + DCBI 180
SA330605205REBOA Zone I + DCBI 180
SA330606126REBOA Zone I + DCBI 180
SA330607167REBOA Zone I + DCBI 180
SA330608192REBOA Zone I + DCBI 240
SA330609143REBOA Zone I + DCBI 240
SA330610168REBOA Zone I + DCBI 240
SA330611127REBOA Zone I + DCBI 240
SA330612206REBOA Zone I + DCBI 240
SA330613188REBOA Zone I + DCBI 30
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Collection:

Collection ID:CO003154
Collection Summary:Healthy adolescent, male Yorkshire swine (average weight 50.0 ± 2.7 kg) were acclimated in the animal facilities for a minimum of 3 days and examined by a veterinarian on the day of experimentation. In brief, on the day of surgery, swine anesthesia was induced via intramuscular injection of ketamine (20.0 mg/kg), xylazine (2.0 mg/kg), and acepromazine (0.2 mg/kg) followed by intubation under direct laryngoscopy. Continuous infusion anesthesia was maintained through a superficial ear vein using propofol (3 mg/kg/h) and fentanyl (3 mcg/kg/h). Lactated Ringer’s solution was administered at a rate of 10 mL/kg/h to overcome insensible losses. Mahurkar catheters (12 Fr) were placed in the femoral arteries and veins for vascular access.
Collection Protocol ID:Animal Care and Use Committee approved this animal study under protocol #1050
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003170
Treatment Summary:the DCBI model included blast traumatic brain injury (bTBI), tissue injury through bilateral open femur fractures, followed by severe fixed pressure targeted hemorrhagic shock (HS). Zone-I aortic occlusion (AO) was performed using the ER-REBOA Catheter (Prytime Medical Devices, Inc., Boerne, TX). The catheter was inserted through a 7 Fr sheath placed in the common femoral artery, was advanced to 50 cm, and inflated using 6 mL of normal saline for Zone-I AO and placed at 26 cm with 5 ml of saline for Zone-III, and both were maintained for the final 30 minutes of the HS phase.

Sample Preparation:

Sampleprep ID:SP003167
Sampleprep Summary:Plasma samples were thawed on ice then 20 μl was aliquoted into individual 1.5mL microcentrifuge tubes. The plasma was diluted to 480 μl with methanol:acetonitrile:water (5:3:2, v/v/v). The metabolite containing supernatant was transferred to individual auto sampler vials on ice.
Sampleprep Protocol Comments:DOI https://doi.org/10.1007/978-1-4939-9236-2_2
Processing Storage Conditions:-80℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN004996 AN004997
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units area under curve area under curve

Chromatography:

Chromatography ID:CH003775
Chromatography Summary:The LC-MS solvents A and B, as well as the Flow gradient details are proprietary and will be made available upon request (angelo.dalesandro@cuanschutz.edu)
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:-
Flow Rate:0.3mL/min
Solvent A:-
Solvent B:-
Chromatography Type:Reversed phase

MS:

MS ID:MS004736
Analysis ID:AN004996
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Scanning in Full MS mode (2 μscans) from 90 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 sheath gas, 15 auxiliary gas(1). Following data acquisition, .raw files were converted to .mzXML using RawConverter then metabolites assigned and peaks integrated using ElMaven (Elucidata) in conjunction with an in-house standard library.
Ion Mode:NEGATIVE
  
MS ID:MS004737
Analysis ID:AN004997
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Scanning in Full MS mode (2 μscans) from 90 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 sheath gas, 15 auxiliary gas(1). Following data acquisition, .raw files were converted to .mzXML using RawConverter then metabolites assigned and peaks integrated using ElMaven (Elucidata) in conjunction with an in-house standard library.
Ion Mode:POSITIVE
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