Summary of Study ST002550
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001642. The data can be accessed directly via it's Project DOI: 10.21228/M8FX54 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002550 |
| Study Title | Targeted metabolomics for ARDS |
| Study Summary | Acute respiratory distress syndrome (ARDS) is a heterogeneous disease in its etiology and clinical aspects, and it has been an important interest in how to diagnose it and classify its subtypes, and apply them to treatment. Metabolomics is becoming important for identifying ARDS biology and distinguishing subtypes. The aim of this study is to identify metabolites distinguishing sepsis-induced ARDS patients from healthy controls using a targeted metabolomics approach, and to find out whether direct and indirect ARDS are metabolically distinct groups and confirm their metabolites and associated pathways. Targeted metabolomics was performed to explore metabolome changes between pARDS (pediatric ARDS) and eARDS. |
| Institute | Asan Medical Center |
| Last Name | Yoo |
| First Name | Hyun Ju |
| Address | 88, Olympic-ro 43-gil, Songpa-gu |
| yoohyunju@amc.seoul.kr | |
| Phone | 0230104029 |
| Submit Date | 2023-03-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004198 | AN004199 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | nM | nM |
Chromatography:
| Chromatography ID: | CH003111 |
| Chromatography Summary: | LC-MS/MS (MRM) for phospholipid |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | isocratic condition of 60% B |
| Flow Rate: | 400 µL/min |
| Solvent A: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(900/50/50) |
| Solvent B: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(940/50/10) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003112 |
| Chromatography Summary: | LC-MS/MS (MRM) for sphingolipid |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 50% A for 0 min, 50% A for 5 min, 50 to 30% A for 3 min, 30% A for 7 min, 30 to 10% A for 7 min, 10% A for 3 min, 10 to 50% A for 0.1 min, and 50% A for 4.9 min |
| Flow Rate: | 200 µL/min |
| Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran(500/200/300) |
| Solvent B: | 5 mM ammonium formate/MeOH/tetrahydrofuran(100/200/700) |
| Chromatography Type: | Reversed phase |
