Summary of Study ST002550

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001642. The data can be accessed directly via it's Project DOI: 10.21228/M8FX54 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002550
Study Title	Targeted metabolomics for ARDS
Study Summary	Acute respiratory distress syndrome (ARDS) is a heterogeneous disease in its etiology and clinical aspects, and it has been an important interest in how to diagnose it and classify its subtypes, and apply them to treatment. Metabolomics is becoming important for identifying ARDS biology and distinguishing subtypes. The aim of this study is to identify metabolites distinguishing sepsis-induced ARDS patients from healthy controls using a targeted metabolomics approach, and to find out whether direct and indirect ARDS are metabolically distinct groups and confirm their metabolites and associated pathways. Targeted metabolomics was performed to explore metabolome changes between pARDS (pediatric ARDS) and eARDS.
Institute	Asan Medical Center
Last Name	Yoo
First Name	Hyun Ju
Address	88, Olympic-ro 43-gil, Songpa-gu
Email	yoohyunju@amc.seoul.kr
Phone	0230104029
Submit Date	2023-03-30
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-07-06
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002656
Sampleprep Summary:	50 μL human serum was mixed well, and 50 μL of internal standard solutions (50 nM C17 ceramide for sphingolipid; 200 nM 18:0 D70 PC and 1 μM 16:0 D31-18:1 PE for plasmalogen and PC/PE profiling) were added. Metabolites were extracted from aqueous and organic phases by liquid-liquid extraction after the addition of chloroform and H2O. Nonpolar metabolites containing lipids were collected from the lower organic phase. The organic phases were dried under vacuum and stored at -20℃oC until further analysis. The dried matter was reconstituted with 50 μL of MeOH prior to LC-MS/MS analysis.

Sampleprep ID:

SP002656

Sampleprep Summary:

50 μL human serum was mixed well, and 50 μL of internal standard solutions (50 nM C17 ceramide for sphingolipid; 200 nM 18:0 D70 PC and 1 μM 16:0 D31-18:1 PE for plasmalogen and PC/PE profiling) were added. Metabolites were extracted from aqueous and organic phases by liquid-liquid extraction after the addition of chloroform and H2O. Nonpolar metabolites containing lipids were collected from the lower organic phase. The organic phases were dried under vacuum and stored at -20℃oC until further analysis. The dried matter was reconstituted with 50 μL of MeOH prior to LC-MS/MS analysis.