Summary of Study ST002772
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001729. The data can be accessed directly via it's Project DOI: 10.21228/M86X4K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002772 |
| Study Title | Day-night fluctuations in Cerebrospinal fluid metabolomics |
| Study Type | Circadian (Naïve animals) |
| Study Summary | This study was designed to investigate whether the molecular drivers underlying the diurnal fluctuation of CSF secretion rate could be found in either the choroid plexus tissue itself and/or in the CSF surrounding it. Male Sprague-Dawley rats, kept in light-controlled 12:12 conditions, presented with diurnally modulated CSF metabolite composition. This change was accompanied with differential expression of 2778 genes within the choroid plexus, with several plasma membrane transporters and CLOCK-associated genes diurnally modulated. |
| Institute | University of Copenhagen |
| Department | Department of Neuroscience |
| Laboratory | MacAulay Group |
| Last Name | Edelbo |
| First Name | Beatriche |
| Address | Blegdamsvej 3B |
| beatriche.henriksen@sund.ku.dk | |
| Phone | +4527697585 |
| Submit Date | 2023-07-04 |
| Num Groups | 2 |
| Total Subjects | 25 |
| Num Males | 25 |
| Study Comments | 2 night animals were excluded due to comments regarding sampling/behavior (N4 and N11) |
| Publications | Day-night fluctuations in choroid plexus transcriptomics and cerebrospinal fluid metabolomics |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004512 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF MS |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003389 |
| Chromatography Summary: | HILIC and Reverse-phase was performed by MS-Omics (Denmark). Data recieved includ raw spectrometry files and combined data from the negative and positive mode (supplied in data section) |
| Chromatography Comments: | For Reverse phase column was Water acquity HSS T3 (2.1x150 mm, 1.7 um) coupled with a high-resolution quadrupole-orbitrap mass spectrometer |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | - |
| Flow Rate: | 300 uL/minute |
| Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid (pH 3.1) |
| Solvent B: | 100% methanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
