Summary of Study ST002874
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001796. The data can be accessed directly via it's Project DOI: 10.21228/M8JX52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002874 |
| Study Title | Quantification of metabolites in kynurenine pathway |
| Study Summary | Urine samples were collected from two independent cohorts. Patients with LN were classified into proliferative (class III/IV) and membranous (class V) by kidney histopathology. Quantification of metabolites in kynurenine pathway was performed using LC-MS/MS. |
| Institute | Mahidol University |
| Department | Siriraj Metabolomics and Phenomics Center |
| Laboratory | Siriraj Center of Research Excellence in Metabolomics and Systems Biology |
| Last Name | Khoomrung |
| First Name | Sakda |
| Address | 2 Wanglang Road Bangkoknoi, Bangkok 10700, Thailand |
| sakda.kho@mahidol.edu | |
| Phone | 024195511 |
| Submit Date | 2023-09-25 |
| Num Groups | 2 |
| Total Subjects | 117 |
| Publications | https://doi.org/10.1016/j.isci.2021.103355 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004711 |
|---|---|
| Analysis type | MS |
| Chromatography type | Normal phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Waters Xevo-TQ-S |
| Ion Mode | POSITIVE |
| Units | ng/ml |
Chromatography:
| Chromatography ID: | CH003547 |
| Chromatography Summary: | A volume of 5 μL of a standard or sample was injected onto an HSS T3 column, 2.1 × 100 mm, 1.8 μM column (Waters, Milford, MA, USA) at 30°C with a constant flow rate of 0.3 mL/min. Mobile phases consisted of (A) 0.1% formic acid in Milli-Q water and (B) 0.1% formic acid in ACN. The gradient program started at 99% A, decreased to 70% over 7.0 min, then decreased to 30% at 9 min, and return to the initial condition at 10 min and held for 4.0 min. The total runtime was 14 min. The weak and strong needles wash solutions were 5% ACN and H2O/ACN/MeOH/IPA (1:1:1:1), respectively. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | The gradient program started at 99% A, decreased to 70% over 7.0 min, then decreased to 30% at 9 min and return to the initial condition at 10 min and held for 4.0 min. The total runtime was 14 min. The weak and strong needles wash solutions were 5% ACN and H2O/ACN/MeOH/IPA (1:1:1:1), respectively. |
| Flow Rate: | 0.4ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Normal phase |
