Summary of Study ST002874
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001796. The data can be accessed directly via it's Project DOI: 10.21228/M8JX52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002874 |
Study Title | Quantification of metabolites in kynurenine pathway |
Study Summary | Urine samples were collected from two independent cohorts. Patients with LN were classified into proliferative (class III/IV) and membranous (class V) by kidney histopathology. Quantification of metabolites in kynurenine pathway was performed using LC-MS/MS. |
Institute | Mahidol University |
Department | Siriraj Metabolomics and Phenomics Center |
Laboratory | Siriraj Center of Research Excellence in Metabolomics and Systems Biology |
Last Name | Khoomrung |
First Name | Sakda |
Address | 2 Wanglang Road Bangkoknoi, Bangkok 10700, Thailand |
sakda.kho@mahidol.edu | |
Phone | 024195511 |
Submit Date | 2023-09-25 |
Num Groups | 2 |
Total Subjects | 117 |
Publications | https://doi.org/10.1016/j.isci.2021.103355 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2023-10-10 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002993 |
Sampleprep Summary: | The urine samples were prepared based on a previously published protocol (Zhu et al., 2019) with minor modifications. Briefly, a 50 μL of each urine sample was mixed with 200 μL of MeOH/ACN (1:1, v/v) containing 100 ng of anthranilic acid C13 (Ant-C13) as an internal standard (IS). The mixture was vortexed for 30 s and sonicated for 10 min (room temperature). The mixture was then left overnight (−20°C) for protein precipitation before centrifugation at 13,000 rpm (4°C) for 15 min. The supernatant was transferred to a new test tube and evaporated to dryness (room temperature) using a vacuum concentrator (Labconco, MO, USA). The dried sample was reconstituted in 100 μL of Milli-Q H2O (containing 0.1% formic acid), vortexed for 30 s, and sonicated (room temperature) for 10 min. The reconstituted sample was centrifuged at 13,000 rpm (4°C) for 15 min. The supernatant was kept at −80°C before analysis. |