Summary of Study ST001399

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000959. The data can be accessed directly via it's Project DOI: 10.21228/M8QX3V This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001399
Study TitleActivation of ectopic olfactory receptor 544 induces GLP-1 secretion, alters gut microbiome, and improves intestinal permeability.
Study TypeFecal metabolome
Study SummaryMetabolome data set from mouse fecal samples Group - WT_AZA: fecal samples from wild type mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - WT_DW: fecal samples from wild type mouse fed with high fat diet Group - KO_AZA: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - KO_DW: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet
Institute
Korea University
Last NameKim
First NameJungyeon
Address145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South
Emailkim131812@korea.ac.kr
Phone821082248015
Submit Date2020-06-07
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2020-06-30
Release Version1
Jungyeon Kim Jungyeon Kim
https://dx.doi.org/10.21228/M8QX3V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001468
Collection Summary:Fecal samples were thawed on ice, and 50 mg of each sample was transferred to a fresh 1.5-mL Eppendorf tube. Then, 1 mL of pure methanol (Merck Millipore, Burlington, MA, USA) was added to each sample. The mixture was homogenised using a needle, sonicated for 30 min, vortexed for 10 min, and centrifuged at 16,100 × g for 10 min at 4°C. The supernatant was filtered by using a syringe filter (0.20-µm pore size; Thermo Fisher Scientific, Waltham, MA, USA). Aliquots of 500 μL of the supernatant were dried under vacuum at room temperature.
Sample Type:Feces
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