Summary of Study ST001399
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000959. The data can be accessed directly via it's Project DOI: 10.21228/M8QX3V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001399 |
| Study Title | Activation of ectopic olfactory receptor 544 induces GLP-1 secretion, alters gut microbiome, and improves intestinal permeability. |
| Study Type | Fecal metabolome |
| Study Summary | Metabolome data set from mouse fecal samples Group - WT_AZA: fecal samples from wild type mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - WT_DW: fecal samples from wild type mouse fed with high fat diet Group - KO_AZA: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - KO_DW: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet |
| Institute | Korea University |
| Last Name | Kim |
| First Name | Jungyeon |
| Address | 145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South |
| kim131812@korea.ac.kr | |
| Phone | 821082248015 |
| Submit Date | 2020-06-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2020-06-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002339 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Arbtrary unit |
MS:
| MS ID: | MS002181 |
| Analysis ID: | AN002339 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | Mass spectra were recorded over a mass range of 85–500 m/z at an acquisition rate of 16 spectra/s. The temperatures of the ion source and transfer line of the TOF–MS were 250°C and 280°C, respectively. The electron ionisation energy was 70 eV.Chroma TOF Software C version (LECO) was used for the detection and deconvolution of peaks and mass spectra. BinBase was used for the identification and relative quantification of metabolites from preprocessed data by matching mass spectra and retention indices of peaks with the customised reference mass spectral and retention index libraries, acquired using authentic standards with identical data acquisition parameters for the Fiehn and NIST libraries. Peaks with mass spectral similarities above 700, in comparison to their authentic standards in the libraries, were regarded as authentic metabolites. Intensities of metabolites were reported as peak heights of their unique ion intensities. For management of missing values, the lowest background intensity was subtracted from the intensity of the quantified ion in its retention time region ± 5s, using MZmine. |
| Ion Mode: | POSITIVE |
