Summary of Study ST001752
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001123. The data can be accessed directly via it's Project DOI: 10.21228/M8JD7N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001752 |
| Study Title | Dual RNA regulator VcdRP in V. cholerae modulates central metabolism |
| Study Summary | Bacterial small RNAs (sRNAs) are well-known to modulate gene expression by base-pairing with trans-coded transcripts and are typically considered to be non-coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual-function regulators. We discovered a dual-function regulator from Vibrio cholerae, called VcdRP, harboring a 29 amino acid protein (VcdP), as well as a base-pairing sequence. In this study, we measured the metabolite abundance of glycolytic and citric acid cycle intermediates using LC-MS. |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Molecular Systems Biology |
| Laboratory | Functional Metabolomics |
| Last Name | Engelmann |
| First Name | Beatrice |
| Address | Permoserstraße 15, Leipzig, Saxony, 03418, Germany |
| beatrice.engelmann@ufz.de | |
| Phone | 00493412351099 |
| Submit Date | 2021-04-14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-05-01 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001822 |
| Collection Summary: | V. cholerae wild-type, ΔvcdRP and ΔgltA each harboring an empty control plasmid (pCtrl) and ΔvcdRP with pVcdRP expression plasmids (pVcdRP and pVcdP) as well as ΔgltA harboring pVcdP were grown in LB medium to exponential and stationary phase, and were subsequently quenched in ice-cold methanol. The quenched samples were centrifuged at 3000 x g for 10 min at -9°C. 1ml aliquot of supernatant was stored for leakage analysis, while the rest of the pellets were flash frozen in liquid nitrogen. |
| Sample Type: | Bacterial cells |
