Summary of Study ST001752
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001123. The data can be accessed directly via it's Project DOI: 10.21228/M8JD7N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001752 |
Study Title | Dual RNA regulator VcdRP in V. cholerae modulates central metabolism |
Study Summary | Bacterial small RNAs (sRNAs) are well-known to modulate gene expression by base-pairing with trans-coded transcripts and are typically considered to be non-coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual-function regulators. We discovered a dual-function regulator from Vibrio cholerae, called VcdRP, harboring a 29 amino acid protein (VcdP), as well as a base-pairing sequence. In this study, we measured the metabolite abundance of glycolytic and citric acid cycle intermediates using LC-MS. |
Institute | Helmholtz Centre for Environmental Research |
Department | Molecular Systems Biology |
Laboratory | Functional Metabolomics |
Last Name | Engelmann |
First Name | Beatrice |
Address | Permoserstraße 15, Leipzig, Saxony, 03418, Germany |
beatrice.engelmann@ufz.de | |
Phone | 00493412351099 |
Submit Date | 2021-04-14 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2021-05-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001835 |
Sampleprep Summary: | The frozen cell pellets were thawed on ice and resuspended in 500 µl methanol. After three freeze (liquid nitrogen) and thaw cycles, the sample was centrifuged (16000 x g, -4 °C and 5 min). The supernatant was transferred into a new tube and stored on ice. The extraction step was repeated and the supernatants were combined prior to evaporation. The samples were stored at -80°C until further analysis. |
Extract Storage: | -80℃ |