Summary of Study ST002968
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001847. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT64 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002968 |
| Study Title | Untargeted metabolomic studies of the PSD95-nNOS uncoupling agent against post-stroke depression in rats |
| Study Type | research |
| Study Summary | To elucidate the antidepressant mechanisms of the PSD95-nNOS decoupler ZL006 using an innovative integrated metabolomics approach. |
| Institute | Nanjing University of Science & Technology |
| Department | Center of Molecular Metabolism |
| Last Name | Hu |
| First Name | Yudie |
| Address | Nanjing University of Science and Technology |
| huyudie@njust.edu.cn | |
| Phone | +8618759270506 |
| Submit Date | 2023-11-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-28 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003074 |
| Collection Summary: | After one week of adaptation (290-320g), the rats underwent middle cerebral artery occlusion (MCAO) surgery to induce focal cerebral ischemia: referring to literature, the thread embolism method was used to induce focal cerebral ischemia for 60 minutes. Rats were evaluated by Longa scoring at 1h and 24h after surgery. Rats with Longa scores of 1-3 were included in subsequent experiments. Penicillin was given daily to the rats for 3 consecutive days after surgery. 3 days later, chronic unpredictable mild stress (CUMS) combined with social isolation modeling method was used to establish the PSD model. The CUMS model included: day-night reversal for 36 h; water deprivation for 18 h; food deprivation for 24h; humid environment for 24 h; 45° inclined cage for 18h; forced swimming in 4°c cold water for 5 min; 45°c stimulation for 5 min; tail pinch for 1 min. Rats were subjected to one of the stimuli randomly at a fixed time every day in an unpredictable way. Each stimulus was used no more than 4 times over 3 weeks. The rats in the Sham group were group-housed with 4-6 rats per cage. Rats that died or underscored during the experiment were replaced by the same batch of rats, the success rate of MCAO surgery was 83%. After 21 days of CUMS, the low-dose and high-dose ZL006 groups received intraperitoneal injection of prepared drug solution (10% ZL006-DMSO solution, 90% ethanol: corn oil: saline = 1:1:8, 2% Tween-80), while the Sham and Model groups received the same volume of solvent. |
| Sample Type: | Brain cortex |
