Summary of Study ST002968

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001847. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT64 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002968
Study Title	Untargeted metabolomic studies of the PSD95-nNOS uncoupling agent against post-stroke depression in rats
Study Type	research
Study Summary	To elucidate the antidepressant mechanisms of the PSD95-nNOS decoupler ZL006 using an innovative integrated metabolomics approach.
Institute	Nanjing University of Science & Technology
Department	Center of Molecular Metabolism
Last Name	Hu
First Name	Yudie
Address	Nanjing University of Science and Technology
Email	huyudie@njust.edu.cn
Phone	+8618759270506
Submit Date	2023-11-08
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-11-28
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003087
Sampleprep Summary:	After euthanizing the rats, the brains were rapidly removed and the brain cortical tissues were separated on ice and stored at -80°C. 30 mg of each sample was added to 450 μL pre-cooled MeOH: H2O (4:1, v/v) and zirconium beads. The homogenizer was pre-cooled and homogenized at 50 Hz for 3 × 15 s with an interval of 5 s. After standing at -20°C for 1 h, samples were centrifuged at 16000 g and 4°C for 15 min. The supernatant was collected, a portion of each brain tissue sample was taken as quality control, the remaining samples and QC were evaporated to 4/5 of the original volume under a nitrogen blower. Excess water was removed using a freeze dryer and stored at -80°C. The samples were reconstituted with 200 μL methanol/water (1:1, v/v). After vortexing for 30 s and ultrasonicating on ice for 1 min, samples were centrifuged at 16000 g and 4°C for 15 min. The supernatant was transferred to UPLC injection vials for LC-MS analysis.

Sampleprep ID:

SP003087

Sampleprep Summary:

After euthanizing the rats, the brains were rapidly removed and the brain cortical tissues were separated on ice and stored at -80°C. 30 mg of each sample was added to 450 μL pre-cooled MeOH: H2O (4:1, v/v) and zirconium beads. The homogenizer was pre-cooled and homogenized at 50 Hz for 3 × 15 s with an interval of 5 s. After standing at -20°C for 1 h, samples were centrifuged at 16000 g and 4°C for 15 min. The supernatant was collected, a portion of each brain tissue sample was taken as quality control, the remaining samples and QC were evaporated to 4/5 of the original volume under a nitrogen blower. Excess water was removed using a freeze dryer and stored at -80°C. The samples were reconstituted with 200 μL methanol/water (1:1, v/v). After vortexing for 30 s and ultrasonicating on ice for 1 min, samples were centrifuged at 16000 g and 4°C for 15 min. The supernatant was transferred to UPLC injection vials for LC-MS analysis.