Summary of Study ST000119
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000106. The data can be accessed directly via it's Project DOI: 10.21228/M8RC75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000119 |
| Study Title | Metabolomics analysis of multiple metabolic functions in the YjgF/YER057c/UK114 (Rid) protein family LCMS |
| Study Type | wildtype vs knock-out |
| Study Summary | Metabolomics analysis of wild-type S. enterica, single RidA knock-out and triple Rid (ridA Rid2 Rid7) knock-out S.enterica cells |
| Institute | University of California, Davis |
| Department | Genome and Biomedical Sciences Facility |
| Laboratory | Fiehn Laboratory |
| Last Name | ElBadawi-Sidhu |
| First Name | Mona |
| Address | 451 Health Sciences Drive, Davis, California 95616, USA |
| mmelbadawi@ucdavis.edu | |
| Submit Date | 2014-11-06 |
| Num Groups | 4 |
| Total Subjects | 6 |
| Study Comments | Samples were measured in poitive and negative ionization mode. MS/MS files contain product ion scans (MS/MS) spectra for compound identification. |
| Raw Data Available | No |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2014-11-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000200 | AN000201 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
MS:
| MS ID: | MS000163 |
| Analysis ID: | AN000200 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3000 |
| Collision Energy: | Ramped 0.02eV/m/z + 10 eV |
| Collision Gas: | Nitrogen |
| Dry Gas Flow: | Nebulizer: 10 L/min Sheath: 9 L/min |
| Dry Gas Temp: | Nebulizer and Sheath: 350 |
| Fragment Voltage: | 125 |
| Ionization: | Positive |
| Precursor Type: | Intact Molecule |
| Reagent Gas: | Nitrogen |
| Dataformat: | .d |
| Nebulizer: | 35 psig |
| Octpole Voltage: | 750 |
| Resolution Setting: | Extended Dynamic Range |
| Scan Range Moverz: | 100 - 1700 m/z |
| Scanning Cycle: | 2 |
| MS ID: | MS000164 |
| Analysis ID: | AN000201 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3000 |
| Collision Energy: | Ramped 0.02eV/m/z + 10 eV |
| Collision Gas: | Nitrogen |
| Dry Gas Flow: | Nebulizer: 10 L/min Sheath: 9 L/min |
| Dry Gas Temp: | Nebulizer and Sheath: 350 |
| Fragment Voltage: | 125 |
| Ionization: | Negative |
| Precursor Type: | Intact Molecule |
| Reagent Gas: | Nitrogen |
| Dataformat: | .d |
| Nebulizer: | 35 psig |
| Octpole Voltage: | 750 |
| Resolution Setting: | Extended Dynamic Range |
| Scan Range Moverz: | 100 - 1700 m/z |
| Scanning Cycle: | 2 |
