Summary of Study ST001148

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000767. The data can be accessed directly via it's Project DOI: 10.21228/M8J68Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001148
Study TitleEffect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts
Study TypeMethod
Study SummaryHuman skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility.
Institute
Mayo Clinic
DepartmentNeurology
Last NameWilkins
First NameJordan
Address200 First St SW
Emailwilkins.jordan@mayo.edu
Phone5072933857
Submit Date2019-02-27
Analysis Type DetailGC/LC-MS
Release Date2019-09-23
Release Version1
Jordan Wilkins Jordan Wilkins
https://dx.doi.org/10.21228/M8J68Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001893 AN001894 AN001895
Analysis type MS MS MS
Chromatography type GC Reversed phase Reversed phase
Chromatography system Agilent 7890B Waters Acquity Waters Acquity
Column Agilent HP5-MS (30m x 0.25mm, 0.25 um) Waters Acquity BEH C18 (150 x 2mm, 1.7um) Waters Acquity BEH C8 (150 x 2.1mm, 1.7um)
MS Type EI ESI ESI
MS instrument type Single quadrupole Triple quadrupole Triple quadrupole
MS instrument name Agilent 5977A Thermo Quantiva Thermo Quantum Ultra
Ion Mode POSITIVE POSITIVE POSITIVE
Units micromol metabolite per gram protein micromol metabolite per gram protein micromol metabolite per gram protein

MS:

MS ID:MS001749
Analysis ID:AN001893
Instrument Name:Agilent 5977A
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:Mass Hunter acquisition B.07, Mass Hunter Quantitation B.08 (Agilent)
Ion Mode:POSITIVE
  
MS ID:MS001750
Analysis ID:AN001894
Instrument Name:Thermo Quantiva
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Xcalibur acquisition and Quantitation 4.0.27 (Thermo Electron)
Ion Mode:POSITIVE
  
MS ID:MS001751
Analysis ID:AN001895
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Xcalibur acquisition and Quantitation 2.0.7 (Thermo Electron)
Ion Mode:POSITIVE
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