Summary of Study ST001148
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000767. The data can be accessed directly via it's Project DOI: 10.21228/M8J68Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001148 |
| Study Title | Effect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts |
| Study Type | Method |
| Study Summary | Human skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility. |
| Institute | Mayo Clinic |
| Department | Neurology |
| Last Name | Wilkins |
| First Name | Jordan |
| Address | 200 First St SW |
| wilkins.jordan@mayo.edu | |
| Phone | 5072933857 |
| Submit Date | 2019-02-27 |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001893 | AN001894 | AN001895 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890B | Waters Acquity | Waters Acquity |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) | Waters Acquity BEH C18 (150 x 2mm,1.7um) | Waters Acquity BEH C8 (150 x 2.1mm,1.7um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | Single quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 5977A | Thermo Quantiva | Thermo Quantum Ultra |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE |
| Units | micromol metabolite per gram protein | micromol metabolite per gram protein | micromol metabolite per gram protein |
MS:
| MS ID: | MS001749 |
| Analysis ID: | AN001893 |
| Instrument Name: | Agilent 5977A |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | Mass Hunter acquisition B.07, Mass Hunter Quantitation B.08 (Agilent) |
| Ion Mode: | POSITIVE |
| MS ID: | MS001750 |
| Analysis ID: | AN001894 |
| Instrument Name: | Thermo Quantiva |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Xcalibur acquisition and Quantitation 4.0.27 (Thermo Electron) |
| Ion Mode: | POSITIVE |
| MS ID: | MS001751 |
| Analysis ID: | AN001895 |
| Instrument Name: | Thermo Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Xcalibur acquisition and Quantitation 2.0.7 (Thermo Electron) |
| Ion Mode: | POSITIVE |
