Summary of Study ST001148

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000767. The data can be accessed directly via it's Project DOI: 10.21228/M8J68Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001148
Study TitleEffect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts
Study TypeMethod
Study SummaryHuman skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility.
Institute
Mayo Clinic
DepartmentNeurology
Last NameWilkins
First NameJordan
Address200 First St SW
Emailwilkins.jordan@mayo.edu
Phone5072933857
Submit Date2019-02-27
Analysis Type DetailGC/LC-MS
Release Date2019-09-23
Release Version1
Jordan Wilkins Jordan Wilkins
https://dx.doi.org/10.21228/M8J68Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000767
Project DOI:doi: 10.21228/M8J68Z
Project Title:Effect of harvesting technique and storage on the metabolic profile of patient-derived skin fibroblasts
Project Type:MS targeted metabolomics
Project Summary:Using human skin fibroblasts, we measured the effect of different cell harvesting techniques (trypsinization, scraping, and methanol fixation) as well as storage time on the metabolic profiles of organic acids, amino acids, and acylcarnitines.
Institute:Mayo Clinic
Department:Neurology
Last Name:Wilkins
First Name:Jordan
Address:200 First St SW
Email:wilkins.jordan@mayo.edu
Phone:5072933857
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