Summary of Study ST001148
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000767. The data can be accessed directly via it's Project DOI: 10.21228/M8J68Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001148 |
| Study Title | Effect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts |
| Study Type | Method |
| Study Summary | Human skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility. |
| Institute | Mayo Clinic |
| Department | Neurology |
| Last Name | Wilkins |
| First Name | Jordan |
| Address | 200 First St SW |
| wilkins.jordan@mayo.edu | |
| Phone | 5072933857 |
| Submit Date | 2019-02-27 |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
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Project:
| Project ID: | PR000767 |
| Project DOI: | doi: 10.21228/M8J68Z |
| Project Title: | Effect of harvesting technique and storage on the metabolic profile of patient-derived skin fibroblasts |
| Project Type: | MS targeted metabolomics |
| Project Summary: | Using human skin fibroblasts, we measured the effect of different cell harvesting techniques (trypsinization, scraping, and methanol fixation) as well as storage time on the metabolic profiles of organic acids, amino acids, and acylcarnitines. |
| Institute: | Mayo Clinic |
| Department: | Neurology |
| Last Name: | Wilkins |
| First Name: | Jordan |
| Address: | 200 First St SW |
| Email: | wilkins.jordan@mayo.edu |
| Phone: | 5072933857 |
