Summary of Study ST001185
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000796. The data can be accessed directly via it's Project DOI: 10.21228/M8SM3C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001185 |
Study Title | Genetic and metabolic characterization of bioengineered human fatty liver tissue with modified SIRT1 expression |
Study Summary | Lipidomics and metabolomics was performed three types of tissue samples to compare human normal liver tissue against human NASH liver and the bioengineered human iPS-derived fatty liver tissue-iKD-SIRT1. The purpose of this study was to show that the global lipidomics profile of iPS-derived fatty liver tissue-iKD-SIRT1 was similar to that of patients with NASH |
Institute | University of Pittsburgh |
Department | Department of Pathology |
Last Name | Soto-Gutierrez |
First Name | Alejandro |
Address | 200 Lothrop Street, 423 Biomedical Science Tower, Pittsburgh, PA 15261, USA |
als208@pitt.edu | |
Phone | +14126480064 |
Submit Date | 2019-05-20 |
Num Groups | 3 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2020-01-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001967 | AN001968 | AN001969 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) | Phenomenex Kinetex C18+ (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | UNSPECIFIED |
Units | Peak area normalized to internal standard | Peak area normalized to internal standard | Peak Area |
MS:
MS ID: | MS001821 |
Analysis ID: | AN001967 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | LIPIDOMICS: Samples were analyzed using full scan accurate mass at a resolution of 70K in positive and negative mode and 17.5K for ddMS2. Thermo Fisher LipidSearch 4.2.2 software was used for peak quantification, alignment, and MS2 identification. Peak areas were normalized to internal standard. |
Ion Mode: | POSITIVE |
MS ID: | MS001822 |
Analysis ID: | AN001968 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | LIPIDOMICS: Samples were analyzed using full scan accurate mass at a resolution of 70K in positive and negative mode and 17.5K for ddMS2. Thermo Fisher LipidSearch 4.2.2 software was used for peak quantification, alignment, and MS2 identification. Peak areas were normalized to internal standard. |
Ion Mode: | NEGATIVE |
MS ID: | MS001823 |
Analysis ID: | AN001969 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | METABOLOMICS: Metabolites were identified in both positive and negative mode by accurate mass (≤ 10 ppm) and retention time. Integrated peak areas were then extracted manually using Quan Browser (Thermo Fisher Xcalibur ver 2.7) and normalized to internal standard. |
Ion Mode: | UNSPECIFIED |