Summary of Study ST001436
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000987. The data can be accessed directly via it's Project DOI: 10.21228/M8410G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001436 |
| Study Title | Transkingdom interactions between Lactobacilli and hepatic mitochondria attenuate western diet induced diabetes |
| Study Type | Supplementation of mice with probiotic bacteria |
| Study Summary | For WD + Microbes 1 and WD + Microbes 3 experiments, C57BL/6 mice were fed western diet or western diet supplemented with Lactobacillus gasseri or Lactobacillus johnsonii for 8 weeks and serum was collected from each mice. For Pooled_1 (control group, western diet only), and Pooled_2 (western diet + Lactobacillus gasseri) groups, serum from 5 mice each was pooled. For Pooled_3 (western diet + Lactobacillus johnsonii) group, serum from 4 mice was pooled. For Pooled_7 (control group, western diet only), Pooled_8 (western diet + Lactobacillus gasseri) and Pooled_9 (western diet + Lactobacillus johnsonii) groups, serum from 6 mice each was pooled. For WD + Microbes 6 experiment, C57BL/6 mice were fed western diet for 8 weeks. Then, one group (n = 5) of mice was supplemented with Lactobacillus gasseri for 12 weeks along with continuation of western diet and serum was collected. For Pooled_10 (control group, western diet only) and Pooled_11 (western diet + Lactobacillus gasseri) groups, serum from 5 mice each was pooled. For GF + WD + LG experiment, C57BL/6 germ free mice were fed western diet or western diet supplemented with Lactobacillus gasseri for 2 weeks. For Pooled_12 (control, western diet only) and Pooled_13 (western diet + Lactobacillus gasseri) groups, serum from 2 mice each was pooled. |
| Institute | Oregon State University |
| Department | Pharmaceutical Sciences |
| Laboratory | Morgun and Shulzhenko |
| Last Name | Morgun |
| First Name | Andriy |
| Address | 203 Pharmacy Bldg |
| andriy.morgun@oregonstate.edu | |
| Phone | 1 541 737 8047 |
| Submit Date | 2020-07-28 |
| Num Groups | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-09-21 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001518 |
| Sampleprep Summary: | Metabolites were extracted with 4 volumes of cold methanol/acetonitrile (1:1, v/v). To precipitate proteins, the samples were incubated for 1h at -20°C. After the samples were centrifuged at 4°C for 15 min at 13,000 rpm, the supernatant was collected and evaporated to dryness in a vacuum concentrator. The dry extracts were then reconstituted in 90 μL of acetonitrile/H2O (1:1, v/v) containing 10 ng/mL CUDA (12-[[(cyclohexylamino)carbonyl] amino]-dodecanoic acid). |
