Summary of Study ST001436

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000987. The data can be accessed directly via it's Project DOI: 10.21228/M8410G This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001436
Study TitleTranskingdom interactions between Lactobacilli and hepatic mitochondria attenuate western diet induced diabetes
Study TypeSupplementation of mice with probiotic bacteria
Study SummaryFor WD + Microbes 1 and WD + Microbes 3 experiments, C57BL/6 mice were fed western diet or western diet supplemented with Lactobacillus gasseri or Lactobacillus johnsonii for 8 weeks and serum was collected from each mice. For Pooled_1 (control group, western diet only), and Pooled_2 (western diet + Lactobacillus gasseri) groups, serum from 5 mice each was pooled. For Pooled_3 (western diet + Lactobacillus johnsonii) group, serum from 4 mice was pooled. For Pooled_7 (control group, western diet only), Pooled_8 (western diet + Lactobacillus gasseri) and Pooled_9 (western diet + Lactobacillus johnsonii) groups, serum from 6 mice each was pooled. For WD + Microbes 6 experiment, C57BL/6 mice were fed western diet for 8 weeks. Then, one group (n = 5) of mice was supplemented with Lactobacillus gasseri for 12 weeks along with continuation of western diet and serum was collected. For Pooled_10 (control group, western diet only) and Pooled_11 (western diet + Lactobacillus gasseri) groups, serum from 5 mice each was pooled. For GF + WD + LG experiment, C57BL/6 germ free mice were fed western diet or western diet supplemented with Lactobacillus gasseri for 2 weeks. For Pooled_12 (control, western diet only) and Pooled_13 (western diet + Lactobacillus gasseri) groups, serum from 2 mice each was pooled.
Institute
Oregon State University
DepartmentPharmaceutical Sciences
LaboratoryMorgun and Shulzhenko
Last NameMorgun
First NameAndriy
Address203 Pharmacy Bldg
Emailandriy.morgun@oregonstate.edu
Phone1 541 737 8047
Submit Date2020-07-28
Num Groups7
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2020-09-21
Release Version1
Andriy Morgun Andriy Morgun
https://dx.doi.org/10.21228/M8410G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000987
Project DOI:doi: 10.21228/M8410G
Project Title:Gut microbiota mediates the interplay between immunity and glucose metabolism
Project Type:preclinical studies
Project Summary:Diabetes, obesity and metabolic disease have reached epidemic proportions. Much research on these diseases has focused on tissues such as adipose, liver, muscle and pancreas. The role of the gut in glucose metabolism is relatively unstudied; however, it can also have a significant effect on systemic glucose control. Residing in the gut is a complex community of microbes, termed microbiota, which are important contributors to immunity and metabolism, frequently mediating cross-talks between these two functions. Changes in the gut microbiota of type 2 diabetes (T2D) patients are directly linked to metabolic dysregulation in the disease. Therefore, the goal of the project is to identify and test microbes and microbial factors involved in regulation of glucose metabolism. For this, microbiota perturbation by western diet followed by global analyses of gut microbiome and host transcriptome combined with causal inference analysis is employed. Newly inferred probiotics are tested in mice fed with western diet followed by metabolomics analysis. This research provides a mechanistic explanation of how probiotics helps disclose mechanisms of T2D as well as will identify protective microbial factors for development of therapy of diabetes.
Institute:Oregon State University
Department:Pharmaceutical Sciences
Laboratory:Morgun and Shulzhenko
Last Name:Andriy
First Name:Morgun
Address:203 Pharmacy Bldg, Corvallis, OR 97331
Email:andriy.morgun@oregonstate.edu
Phone:1 541 737 8047
Funding Source:NIH R01 DK103761
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