Summary of Study ST001948
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001235. The data can be accessed directly via it's Project DOI: 10.21228/M82T49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001948 |
| Study Title | Metabolites Associated with Gestational Diabetes in Plasma |
| Study Type | Case:Control ancillary analysis of RCT |
| Study Summary | Gestational diabetes mellitus (GDM) significantly increases maternal and fetal health risks, but factors predictive of GDM are poorly understood. Plasma metabolomics analyses were conducted in early pregnancy to identify potential biomarkers for early prediction of Gestational Diabetes Mellitus (GDM). Sixty-eight pregnant women with overweight/obesity from a clinical trial of a lifestyle intervention were included. Participants who developed GDM (n=34; GDM group) were matched on treatment group, age, body mass index, and ethnicity with those who did not develop GDM (n=34; Non-GDM group). Blood draws were completed early in pregnancy (10-16 weeks). Plasma samples were analyzed by UPLC-MS using three metabolomics assays. |
| Institute | California Polytechnic State University |
| Department | Food Science and Nutrition |
| Laboratory | Cal Poly Metabolomics Service Center |
| Last Name | La Frano |
| First Name | Michael |
| Address | CALIFORNIA POLYTECHNIC STATE UNIVERSITY, 1 GRAND AVE |
| mlafrano@calpoly.edu | |
| Phone | 18057566233 |
| Submit Date | 2021-10-13 |
| Num Groups | 2 |
| Total Subjects | 68 |
| Num Females | 68 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-11-04 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002032 |
| Sampleprep Summary: | 1. 25 µL of plasma were added to 1.5 mL tubes before the addition of 10 µL of 1 µM internal standard solution, followed by 750 µL chilled methanol. 2. Samples were vortexed 30 seconds prior to being centrifuged at 15,000 x G for 10 min. 3. The supernatant was transferred to 1.5 mL high performance liquid chromatography (HPLC) amber glass vials, dried by centrifugal vacuum evaporation, and reconstituted in 100 µL 3:1 acetonitrile:methanol solution with an internal standard [12-[(cyclohexylamino) carbonyl]amino]-dodecanoic acid (CUDA)] solution. 4.The reconstituted solution was vortexed 30 seconds and placed on ice for 10 minutes. 5. The solution was then centrifuged at 10,000 x G for 3 minutes after being transferred to microfilter tubes. 6. The supernatant was transferred to a HPLC vial to be analyzed using the UPLC-MS. |
| Sampleprep Protocol Filename: | La_Frano_Lab_Methods_Doc_v8.pdf |
