Summary of Study ST001948

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001235. The data can be accessed directly via it's Project DOI: 10.21228/M82T49 This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001948
Study TitleMetabolites Associated with Gestational Diabetes in Plasma
Study TypeCase:Control ancillary analysis of RCT
Study SummaryGestational diabetes mellitus (GDM) significantly increases maternal and fetal health risks, but factors predictive of GDM are poorly understood. Plasma metabolomics analyses were conducted in early pregnancy to identify potential biomarkers for early prediction of Gestational Diabetes Mellitus (GDM). Sixty-eight pregnant women with overweight/obesity from a clinical trial of a lifestyle intervention were included. Participants who developed GDM (n=34; GDM group) were matched on treatment group, age, body mass index, and ethnicity with those who did not develop GDM (n=34; Non-GDM group). Blood draws were completed early in pregnancy (10-16 weeks). Plasma samples were analyzed by UPLC-MS using three metabolomics assays.
California Polytechnic State University
DepartmentFood Science and Nutrition
LaboratoryCal Poly Metabolomics Service Center
Last NameLa Frano
First NameMichael
Submit Date2021-10-13
Num Groups2
Total Subjects68
Num Females68
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-11-04
Release Version1
Michael La Frano Michael La Frano application/zip

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:SP002032
Sampleprep Summary:1. 25 µL of plasma were added to 1.5 mL tubes before the addition of 10 µL of 1 µM internal standard solution, followed by 750 µL chilled methanol. 2. Samples were vortexed 30 seconds prior to being centrifuged at 15,000 x G for 10 min. 3. The supernatant was transferred to 1.5 mL high performance liquid chromatography (HPLC) amber glass vials, dried by centrifugal vacuum evaporation, and reconstituted in 100 µL 3:1 acetonitrile:methanol solution with an internal standard [12-[(cyclohexylamino) carbonyl]amino]-dodecanoic acid (CUDA)] solution. 4.The reconstituted solution was vortexed 30 seconds and placed on ice for 10 minutes. 5. The solution was then centrifuged at 10,000 x G for 3 minutes after being transferred to microfilter tubes. 6. The supernatant was transferred to a HPLC vial to be analyzed using the UPLC-MS.
Sampleprep Protocol Filename:La_Frano_Lab_Methods_Doc_v8.pdf