Summary of Study ST001211
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000814. The data can be accessed directly via it's Project DOI: 10.21228/M8G40M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001211 |
| Study Title | Metabolomic Markers of Methotrexate Response, In Vitro |
| Study Summary | Erythroblastoid cells (K562) maintained in logarithmic growth phase were treated with 1000 nM methotrexate or vehicle alone (i.e. D-PBS) under standard culture conditions for 24 hours. Cellular response to methotrexate was measured based on anti-proliferative activity by cell counting. Cells were washed, flash frozen in liquid nitrogen and submitted for metabolomics analysis to the NIH West Coast Metabolomics Center. |
| Institute | University of Kansas |
| Department | Pharmacy Practice |
| Laboratory | Funk Lab |
| Last Name | Funk |
| First Name | Ryan |
| Address | 2106 Olathe Boulevard |
| ryanfunk@kumc.edu | |
| Phone | 9135885000 |
| Submit Date | 2019-07-03 |
| Num Groups | 2 |
| Total Subjects | 20 samples |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2020-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002016 | AN002017 | AN002018 | AN002019 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | GC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Leco Pegasus IV GC | Agilent 6530 | Agilent 6530 | Agilent 6530 |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | EI | ESI | ESI | ESI |
| MS instrument type | GC-TOF | QTOF | QTOF | QTOF |
| MS instrument name | Leco Pegasus IV TOF | Agilent 6530 QTOF | Agilent 6530 QTOF | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE | POSITIVE | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH001458 |
| Chromatography Summary: | Primary Metabolism |
| Instrument Name: | Leco Pegasus IV GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
| Chromatography ID: | CH001459 |
| Chromatography Summary: | Biogenic Amines |
| Instrument Name: | Agilent 6530 |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001460 |
| Chromatography Summary: | Lipidomics |
| Instrument Name: | Agilent 6530 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
