Summary of Study ST001211

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000814. The data can be accessed directly via it's Project DOI: 10.21228/M8G40M This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST001211
Study TitleMetabolomic Markers of Methotrexate Response, In Vitro
Study SummaryErythroblastoid cells (K562) maintained in logarithmic growth phase were treated with 1000 nM methotrexate or vehicle alone (i.e. D-PBS) under standard culture conditions for 24 hours. Cellular response to methotrexate was measured based on anti-proliferative activity by cell counting. Cells were washed, flash frozen in liquid nitrogen and submitted for metabolomics analysis to the NIH West Coast Metabolomics Center.
University of Kansas
DepartmentPharmacy Practice
LaboratoryFunk Lab
Last NameFunk
First NameRyan
Address2106 Olathe Boulevard
Submit Date2019-07-03
Num Groups2
Total Subjects20 samples
Analysis Type DetailGC-MS/LC-MS
Release Date2020-01-06
Release Version1
Ryan Funk Ryan Funk application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN002016 AN002017 AN002018 AN002019
Analysis type MS MS MS MS
Chromatography type GC HILIC Reversed phase Reversed phase
Chromatography system Leco Pegasus IV GC Agilent 6530 Agilent 6530 Agilent 6530
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS instrument type GC-TOF QTOF QTOF QTOF
MS instrument name Leco Pegasus IV TOF Agilent 6530 QTOF Agilent 6530 QTOF Agilent 6550 QTOF
Units Peak height Peak height Peak height Peak height


Chromatography ID:CH001458
Chromatography Summary:Primary Metabolism
Instrument Name:Leco Pegasus IV GC
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:GC
Chromatography ID:CH001459
Chromatography Summary:Biogenic Amines
Instrument Name:Agilent 6530
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Chromatography Type:HILIC
Chromatography ID:CH001460
Chromatography Summary:Lipidomics
Instrument Name:Agilent 6530
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:65
Flow Rate:0.6 mL/min
Solvent A:60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase