Summary of Study ST001211
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000814. The data can be accessed directly via it's Project DOI: 10.21228/M8G40M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001211 |
Study Title | Metabolomic Markers of Methotrexate Response, In Vitro |
Study Summary | Erythroblastoid cells (K562) maintained in logarithmic growth phase were treated with 1000 nM methotrexate or vehicle alone (i.e. D-PBS) under standard culture conditions for 24 hours. Cellular response to methotrexate was measured based on anti-proliferative activity by cell counting. Cells were washed, flash frozen in liquid nitrogen and submitted for metabolomics analysis to the NIH West Coast Metabolomics Center. |
Institute | University of Kansas |
Department | Pharmacy Practice |
Laboratory | Funk Lab |
Last Name | Funk |
First Name | Ryan |
Address | 2106 Olathe Boulevard |
ryanfunk@kumc.edu | |
Phone | 9135885000 |
Submit Date | 2019-07-03 |
Num Groups | 2 |
Total Subjects | 20 samples |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2020-01-06 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001286 |
Sampleprep Summary: | Cell pellets were sent to the West Coast Metabolomics Center and were processed on laboratory Standard Operating Procedures for Sample Preparation of Blood Plasma or Serum Samples for Lipidomic, Biogenic Amine, and Primary Metabolomic Analysis using a biphasic MeOH/MTBE/Water extraction procedure. The upper organic phase was used for lipidomics analysis and the aqueous phase was used for analysis of primary metabolism and biogenic amines. |