Summary of Study ST001211

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000814. The data can be accessed directly via it's Project DOI: 10.21228/M8G40M This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001211
Study TitleMetabolomic Markers of Methotrexate Response, In Vitro
Study SummaryErythroblastoid cells (K562) maintained in logarithmic growth phase were treated with 1000 nM methotrexate or vehicle alone (i.e. D-PBS) under standard culture conditions for 24 hours. Cellular response to methotrexate was measured based on anti-proliferative activity by cell counting. Cells were washed, flash frozen in liquid nitrogen and submitted for metabolomics analysis to the NIH West Coast Metabolomics Center.
University of Kansas
DepartmentPharmacy Practice
LaboratoryFunk Lab
Last NameFunk
First NameRyan
Address2106 Olathe Boulevard
Submit Date2019-07-03
Num Groups2
Total Subjects20 samples
Analysis Type DetailGC-MS/LC-MS
Release Date2020-01-06
Release Version1
Ryan Funk Ryan Funk application/zip

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Sample Preparation:

Sampleprep ID:SP001286
Sampleprep Summary:Cell pellets were sent to the West Coast Metabolomics Center and were processed on laboratory Standard Operating Procedures for Sample Preparation of Blood Plasma or Serum Samples for Lipidomic, Biogenic Amine, and Primary Metabolomic Analysis using a biphasic MeOH/MTBE/Water extraction procedure. The upper organic phase was used for lipidomics analysis and the aqueous phase was used for analysis of primary metabolism and biogenic amines.