Summary of Study ST001211

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000814. The data can be accessed directly via it's Project DOI: 10.21228/M8G40M This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001211
Study TitleMetabolomic Markers of Methotrexate Response, In Vitro
Study SummaryErythroblastoid cells (K562) maintained in logarithmic growth phase were treated with 1000 nM methotrexate or vehicle alone (i.e. D-PBS) under standard culture conditions for 24 hours. Cellular response to methotrexate was measured based on anti-proliferative activity by cell counting. Cells were washed, flash frozen in liquid nitrogen and submitted for metabolomics analysis to the NIH West Coast Metabolomics Center.
University of Kansas
DepartmentPharmacy Practice
LaboratoryFunk Lab
Last NameFunk
First NameRyan
Address2106 Olathe Boulevard
Submit Date2019-07-03
Num Groups2
Total Subjects20 samples
Analysis Type DetailGC-MS/LC-MS
Release Date2020-01-06
Release Version1
Ryan Funk Ryan Funk application/zip

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Treatment ID:TR001293
Treatment Summary:Cells were maintained under normal growth conditions in a humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2. Cells were kept at density between 2.0x105 and 1.0x106 cells/mL to maintain logarithmic growth conditions to promote maximum proliferative activity over the experimental period. All experiments were conducted within six passages following removal from cryopreservation. On the day of experimentation, K562 cells were seeded at a density of 4.0x105 cells/mL and exposed to 1000 nM MTX or D-PBS (i.e. vehicle) for 24 hours under normal growth conditions.
Treatment Compound:Methotrexate
Treatment Route:In Media
Treatment Dose:1000 nM
Treatment Doseduration:24 hours
Treatment Vehicle:Dulbecco's Phosphate Buffered Saline (without Calcium/Magnesium)
Cell Storage:Liquid Nitrogen
Cell Growth Container:T-175 Flask
Cell Growth Config:Suspension
Cell Inoc Proc:Seeded at 4.010^5 cells/mL
Cell Media:RPMI 1640 medium (catalog no. 61870-127, ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (catalog no. S11150, Atlanta Biologicals, Lawrenceville, GA) along with 100 units/mL penicillin and 1,000 units/mL streptomycin (catalog no. 15140122, ThermoFisher Scientific, Waltham, MA)
Cell Envir Cond:humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2
Cell Harvesting:Counted and a volume containing 15x10^6 cells were collected, washed, flash frozen in liquid nitrogen
Cell Pct Confluence:NA
Cell Media Lastchanged:24 hours prior to collection